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Titolo:
In vivo genotoxicity of 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline in lacI transgenic (Big Blue (R)) mice
Autore:
Itoh, T; Suzuki, T; Nishikawa, A; Furukawa, F; Takahashi, M; Xue, W; Sofuni, T; Hayashi, M;
Indirizzi:
Natl Inst Hlth Sci, Div Genet & Mutagenesis, Setagaya Ku, Tokyo 1588501, Japan Natl Inst Hlth Sci Tokyo Japan 1588501 Setagaya Ku, Tokyo 1588501, Japan Natl Inst Hlth Sci, Div Pathol, Setagaya Ku, Tokyo 1588501, Japan Natl Inst Hlth Sci Tokyo Japan 1588501 Setagaya Ku, Tokyo 1588501, Japan
Titolo Testata:
MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS
fascicolo: 1, volume: 468, anno: 2000,
pagine: 19 - 25
SICI:
1383-5718(20000622)468:1<19:IVGO2I>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
MOUSE MUTATION ASSAYS; HETEROCYCLIC AMINES; FOOD MUTAGENS; DNA-ADDUCTS; INVIVO; MEIQX; LIVER; 2-AMINO-1-METHYL-6-PHENYLIMIDAZO<4,5-B>PYRIDINE; DIMETHYLNITROSAMINE; CARCINOGENICITY;
Keywords:
2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline (MeIQx); lacI; Big Blue (R) mouse; micronucleus assay; cell proliferation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Suzuki, T Natl Inst Hlth Sci, Div Genet & Mutagenesis, Setagaya Ku, 1-18-1Kamiyoga,Tokyo 1588501, Japan Natl Inst Hlth Sci 1-18-1 Kamiyoga Tokyo Japan 1588501 1, Japan
Citazione:
T. Itoh et al., "In vivo genotoxicity of 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline in lacI transgenic (Big Blue (R)) mice", MUT RES-GTE, 468(1), 2000, pp. 19-25

Abstract

2-Amino-3,8-dimethylimidazo[4,5-f]quinoxaline (MeIQx), a heterocyclic amine found in cooked meat, is a strong mutagen in the Salmonella/microsome assay and was proven to be a hepatocarcinogen in rodents. We used the lad transgenic (Big Blue(R)) mouse to investigate MeIQx genotoxicity in vivo, lad mutant frequencies were examined in liver and colon after single intragastric administration of MeIQx (males) or 12 weeks of feeding in the diet (malesand females). Micronucleus induction was monitored in the peripheral bloodand cell proliferating activity was monitored by proliferating cell nuclear antigen (PCNA) immunostaining, but only after the intragastric administration. Intragastric treatment with MeIQx (100 mg/kg) did not increase mutantfrequency (MF) in liver or colon but it did induce a slight but statistically significant increase in the incidence of micronucleated reticulocytes 48 h after the treatment. No apparent increase in PCNA-positive foci was observed in any of tissues analyzed 14 days after the treatment. Administration of MeIQx (300 ppm) in diet for 12 weeks, however, caused MF increases in liver and colon in male and female mice, with greater increases in the females. An increase was also obvious after 4 weeks, but only in females. The sex difference in MF is consistent with the fact that female mice are more susceptible to MeIQx carcinogenesis. These results demonstrated that in the transgenic mouse mutation assay, long-term feeding of MeIQx was more effective than single gastric exposures in revealing the compound's mutagenicity in the target organs of carcinogenicity and that sex differences in susceptibility can also be observed. (C) 2000 Elsevier Science B,V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 09:24:13