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Titolo:
Early induction of the orphan nuclear receptor NOR-1 during cell death of the human breast cancer cell line MCF-7
Autore:
Ohkubo, T; Ohkura, N; Maruyama, K; Sasaki, K; Nagasaki, K; Hanzawa, H; Tsukada, T; Yamaguchi, K;
Indirizzi:
Natl Canc Ctr, Inst Res, Div Growth Factor, Chuo Ku, Tokyo 1040045, Japan Natl Canc Ctr Tokyo Japan 1040045 Factor, Chuo Ku, Tokyo 1040045, Japan
Titolo Testata:
MOLECULAR AND CELLULAR ENDOCRINOLOGY
fascicolo: 1-2, volume: 162, anno: 2000,
pagine: 151 - 156
SICI:
0303-7207(20000425)162:1-2<151:EIOTON>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRANSCRIPTION FACTOR; NGFI-B; SOMATOSTATIN GENE; DNA FRAGMENTATION; DEVELOPING BRAIN; NUR77; EXPRESSION; APOPTOSIS; RAT; SUPERFAMILY;
Keywords:
neuron-derived orphan receptor; A23187; apoptosis; cAMP response element;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Ohkura, N Natl Canc Ctr, Inst Res, Div Growth Factor, Chuo Ku, 5-1-1 Tsukiji, Tokyo 1040045, Japan Natl Canc Ctr 5-1-1 Tsukiji Tokyo Japan 1040045 1040045, Japan
Citazione:
T. Ohkubo et al., "Early induction of the orphan nuclear receptor NOR-1 during cell death of the human breast cancer cell line MCF-7", MOL C ENDOC, 162(1-2), 2000, pp. 151-156

Abstract

The neuron-derived orphan receptor (NOR-I) is a member of the NGFI-B subfamily within the nuclear receptor superfamily. In T-cell apoptosis, where NGFI-B plays an essential role, a functional redundancy between NGFI-B and NOR-I has been demonstrated. Here, we examined the regulation and expression of the NOR-1 gene during cell death induced by a calcium ionophore A23187 in the human breast cancer cell line MCF-7. A23187 caused a transient increase in NOR-I mRNA levels within 6 h after treatment. To delineate the sequences required for the transitional response to A23187, a series of promoter deletion mutants were constructed. From the transient transfection experiments, the element responsive to A23187 was identified between - 94 and - 42 base pairs upstream from the transcription initiation site. This 53-base pairs region contains three copies of the cAMP response element (CRE). Furthermore, phosphorylation of the CRE-binding protein (CREB), which affects thetranscription of the CRE dependent-genes, was detected 30 min after A23187stimulation. Our findings are consistent with NOR-1 involvement in A23187-induced cell death via the CRE-CREB signaling pathway. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 16:09:32