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Titolo:
Single-unit and polygraphic recordings associated with systemic or local pharmacology: A multi-purpose stereotaxic approach for the awake, anaesthetic-free, and head-restrained rat
Autore:
Souliere, F; Urbain, N; Gervasoni, D; Schmitt, P; Guillemort, C; Fort, P; Renaud, B; Luppi, PH; Chouvet, G;
Indirizzi:
Univ Lyon 1, INSERM, U512, Lab Neuropharmacol & Neurochim, F-69373 Lyon 8,France Univ Lyon 1 Lyon France 8 ropharmacol & Neurochim, F-69373 Lyon 8,France Univ Lyon 18, INSERM, U480, Lab Neurobiol Sommeil & Eveil, Lyon, France Univ Lyon 18 Lyon France 0, Lab Neurobiol Sommeil & Eveil, Lyon, France GFG Co, F-69491 Pierre Benite, France GFG Co Pierre Benite France F-69491 FG Co, F-69491 Pierre Benite, France
Titolo Testata:
JOURNAL OF NEUROSCIENCE RESEARCH
fascicolo: 1, volume: 61, anno: 2000,
pagine: 88 - 100
SICI:
0360-4012(20000701)61:1<88:SAPRAW>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
LOCUS-COERULEUS NEURONS; VENTRAL TEGMENTAL AREA; GLUTAMATE-INDUCED EXCITATIONS; BRAIN NORADRENERGIC NEURONS; UNRESTRAINED RATS; SUBSTANTIA NIGRA; DOPAMINE NEURONS; MOVING RATS; UNANESTHETIZED RATS; ELECTROPHYSIOLOGICAL EVIDENCE;
Keywords:
unanaesthetized rat; stereotaxic head-restraint; single-unit recordings; vigilance states monitoring; microiontophoresis; micropressure drug applications; systemic pharmacology;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
74
Recensione:
Indirizzi per estratti:
Indirizzo: Chouvet, G Univ Lyon 1, INSERM, U512, Lab Neuropharmacol & Neurochim, 8 Ave Rockefeller, F-69373 Lyon 8, France Univ Lyon 1 8 Ave Rockefeller Lyon France 8 373 Lyon 8, France
Citazione:
F. Souliere et al., "Single-unit and polygraphic recordings associated with systemic or local pharmacology: A multi-purpose stereotaxic approach for the awake, anaesthetic-free, and head-restrained rat", J NEUROSC R, 61(1), 2000, pp. 88-100

Abstract

In order to avoid any artifactual pharmacological interferences with anaesthetic agents, a procedure has been developed for working on the awake, anaesthetic-free rat in a head-restrained condition. It allows, on the same animal and over several consecutive days, single-unit recordings in combination with systemic or local pharmacology (microiontophoresis or micropressureejections), as well as monitoring vigilance states via the electroencephalogram and the electromyogram. After the cementing of a special "U"-shaped device on its skull under general anaesthesia, the animal is progressively habituated to stay daily, for several hours, under a painless corresponding stereotaxic restraint. This system can be easily adapted to different stereotaxic frames and, because of its spatial flexibility for targetting the desired rostrocaudal or lateral positions, allows access to a large number ofcerebral structures. Experiments performed on Globus Pallidus, Substantia Nigra, and Locus Coeruleus neurons, combining the different possibilities of this system, are reported. They demonstrate, on the awake anaesthetic-free head-restrained rat, and under suitable ethical conditions, the feasibility of single-unit recordings of identified neurons associated with the study of their pharmacological reactivity after systemic or local drug administrations without any other drug interferences, and in physiologically relevant conditions such as the spontaneous alternance of vigilance states. J. Neurosci. Res. 61:88-100, 2000. (C) 2000 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/09/20 alle ore 07:37:07