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Titolo:
Differential expression of the G protein beta(5) gene: Analysis of mouse brain, peripheral tissues, and cultured cell lines
Autore:
Zhang, JH; Lai, ZN; Simonds, WF;
Indirizzi:
NIDDKD, Metab Dis Branch, NIH, Bethesda, MD 20892 USA NIDDKD Bethesda MD USA 20892 etab Dis Branch, NIH, Bethesda, MD 20892 USA NIDDKD, Genet Branch, NIH, Bethesda, MD 20892 USA NIDDKD Bethesda MD USA 20892 D, Genet Branch, NIH, Bethesda, MD 20892 USA NIDDKD, Biochem Branch, NIH, Bethesda, MD 20892 USA NIDDKD Bethesda MD USA 20892 Biochem Branch, NIH, Bethesda, MD 20892 USA
Titolo Testata:
JOURNAL OF NEUROCHEMISTRY
fascicolo: 1, volume: 75, anno: 2000,
pagine: 393 - 403
SICI:
0022-3042(200007)75:1<393:DEOTGP>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
ADULT-RAT BRAIN; RGS PROTEINS; GAMMA-SUBUNIT; REGULATORS; COMPLEX; LOCALIZATION; ACTIVATION; RECEPTORS; G-BETA-5; PATHWAYS;
Keywords:
signal transduction; regulator of G protein signaling proteins; effectors;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Simonds, WF NIDDKD, Metab Dis Branch, NIH, Bldg 10,Room 8C-101,10 Ctr Dr,MSC 1752, Bethesda, MD 20892 USA NIDDKD Bldg 10,Room 8C-101,10 Ctr Dr,MSC 1752 Bethesda MD USA 20892
Citazione:
J.H. Zhang et al., "Differential expression of the G protein beta(5) gene: Analysis of mouse brain, peripheral tissues, and cultured cell lines", J NEUROCHEM, 75(1), 2000, pp. 393-403

Abstract

A neurally expressed heterotrimeric G protein beta subunit, G beta(5), hasbeen found to exhibit functional specialization with respect to its interactions with effector targets and G alpha subunits. A splice Variant of G beta(5) that contains an N-terminal 42-residue extension, G beta(5)-long, hasbeen described in the retina. To define better the potential range of its specialized interactions, analysis of G beta(5) gene transcript and proteinexpression in mouse brain and other tissues and cell lines was performed, Quantification by ribonuclease protection assay of G beta(5) transcript expression in the developing brain demonstrates a fivefold increase that occurs postnatally. Analysis of transcript expression by in situ hybridization and ribonuclease protection assay indicates that the G beta(5) gene is differentially expressed among multiple adult mouse brain regions, including themotor and occipital cortex, the olfactory bulb and associated rhinencephalic structures, hypothalamus, pontine cochlear nuclei, and Purkinje cells inthe cerebellum, G beta(5) is also expressed in several cultured cell linesof neuroendocrine origin, including murine alpha T3-1 pituitary gonadotrophs and GT1-7 hypothalamic cells, and rat PC12 pheochromocytoma cells. Immunoblotting of tissue homogenates with antibodies to two peptides common to Gbeta(5) and G beta(5)-long confirmed expression of G beta(5) in multiple brain regions and in spinal cord and expression of G beta(5)-long in retina. Taken together, these results suggest that the specialized molecular properties of G beta(5) have been adapted to diverse neural functions in the adult brain.

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Documento generato il 25/01/21 alle ore 02:31:43