Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Identification of the RecA protein-loading domain of RecBCD enzyme
Autore:
Churchill, JJ; Kowalczykowski, SC;
Indirizzi:
Univ Calif Davis, Microbiol Sect, Grad Grp Biochem & Mol Biol, Davis, CA 95616 USA Univ Calif Davis Davis CA USA 95616 ochem & Mol Biol, Davis, CA 95616 USA Univ Calif Davis, Sect Mol & Cellular Biol, Grad Grp Biochem & Mol Biol, Davis, CA 95616 USA Univ Calif Davis Davis CA USA 95616 ochem & Mol Biol, Davis, CA 95616 USA
Titolo Testata:
JOURNAL OF MOLECULAR BIOLOGY
fascicolo: 3, volume: 297, anno: 2000,
pagine: 537 - 542
SICI:
0022-2836(20000331)297:3<537:IOTRPD>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
ESCHERICHIA-COLI RECA; SINGLE-STRANDED-DNA; RECOMBINATION HOTSPOT; SERRATIA-MARCESCENS; NUCLEASE ACTIVITY; SSB PROTEIN; CHI; PURIFICATION; MIRABILIS; BINDING;
Keywords:
RecBCD enzyme; RecA protein; Chi sequence; recombination; helicase;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
24
Recensione:
Indirizzi per estratti:
Indirizzo: Kowalczykowski, SC Univ Calif Davis, Microbiol Sect, Grad Grp Biochem & Mol Biol, Davis, CA 95616 USA Univ Calif Davis Davis CA USA 95616 avis, CA 95616 USA
Citazione:
J.J. Churchill e S.C. Kowalczykowski, "Identification of the RecA protein-loading domain of RecBCD enzyme", J MOL BIOL, 297(3), 2000, pp. 537-542

Abstract

Genetic recombination in Escherichia coli is stimulated by the recombination hotspot Chi (chi), a regulatory element that modifies the activities of the RecBCD enzyme and leads to loading of the DNA strand exchange protein, RecA, onto the chi-containing DNA strand. The RecBC enzyme, which lacks theRecD subunit, loads RecA protein constitutively, in a manner that is independent of chi. Using a truncated RecBC enzyme lacking the 30 kDa C-terminaldomain of the RecB subunit, we show that this domain is necessary for RecAprotein-loading. We propose that this domain harbors a site that interactswith RecA protein, recruiting it to single-stranded DNA during unwinding. This ability of a translocating enzyme to deliver material (RecA protein) to a specific target site (the chi sequence) parallels that of other cellular motor proteins. (C) 2000 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 23:07:27