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Titolo:
Simultaneous determination of risperidone and 9-hydroxyrisperidone in plasma by liquid chromatography/electrospray tandem mass spectrometry
Autore:
Aravagiri, M; Marder, SR;
Indirizzi:
Univ Calif Los Angeles, VA Greater Los Angeles Healthcare Syst, Psychopharmacol Unit, Los Angeles, CA 90073 USA Univ Calif Los Angeles Los Angeles CA USA 90073 Los Angeles, CA 90073 USA
Titolo Testata:
JOURNAL OF MASS SPECTROMETRY
fascicolo: 6, volume: 35, anno: 2000,
pagine: 718 - 724
SICI:
1076-5174(200006)35:6<718:SDORA9>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
ELECTROCHEMICAL DETECTION; SCHIZOPHRENIC-PATIENTS; PERFORMANCE; METABOLISM; EXCRETION;
Keywords:
risperidone; 9-hydroxyrisperidone; liquid chromatography/electrospray tandem mass spectrometry; plasma; pharmacokinetics;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Physical, Chemical & Earth Sciences
Citazioni:
18
Recensione:
Indirizzi per estratti:
Indirizzo: Aravagiri, M Univ Calif Los Angeles, VA Greater Los Angeles Healthcare Syst, Psychopharmacol Unit, 11301 Wilshire Blvd, Los Angeles, CA 90073 USA Univ Calif Los Angeles 11301 Wilshire Blvd Los Angeles CA USA 90073
Citazione:
M. Aravagiri e S.R. Marder, "Simultaneous determination of risperidone and 9-hydroxyrisperidone in plasma by liquid chromatography/electrospray tandem mass spectrometry", J MASS SPEC, 35(6), 2000, pp. 718-724

Abstract

A simple and highly sensitive liquid chromatographic/electrospray tandem mass spectrometric (LC/MS/MS) assay was developed for the simultaneous determination of risperidone (RSP) and its major circulating metabolite 9-hydroxyrisperidone (9-OH-RSP) in the plasma of humans and rats, A simple one-stepsolvent extraction with 15% methylene chloride in pentane was used to isolate the compounds from plasma. The compounds were eluted from a phenyl-hexyl column and detected with a Perkin-Elmer SCIEX API2000 triple-quadrupole mass spectrometer using positive ion atmospheric pressure electrospray ionization and multiple reaction monitoring. The assay was linear over the range0.1-100 ng ml(-1) when 0.5 ml of plasma was used in the extraction. The overall intra- (within-day) and inter- (between days) assay variations were <11%. The variations in the concentrations of two long-term quality control samples from pooled patient plasma samples analyzed over a period of 6 months were similar to 10%. The analysis time for each sample was 4 min and more than 100 samples could be analyzed in one day by running the system overnight. The assay is simple, highly sensitive, selective, precise and fast, This method is being used for the therapeutic drug monitoring of schizophrenic patients treated with RSP and to study the pharmacokinetics and tissue distribution of RSP and 9-OH-RSP in rats. Copyright (C) 2000 John Wiley Br Sons, Ltd.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/01/20 alle ore 13:58:23