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Titolo:
Integration of laboratory exercises in developmental biology and neurobiology courses using the Xenopus oocyte expression system
Autore:
Silver, WL; Browne, CL;
Indirizzi:
Wake Forest Univ, Dept Biol, Winston Salem, NC 27106 USA Wake Forest UnivWinston Salem NC USA 27106 , Winston Salem, NC 27106 USA
Titolo Testata:
JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
fascicolo: 5, volume: 24, anno: 2000,
pagine: 353 - 358
SICI:
1367-5435(200005)24:5<353:IOLEID>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Keywords:
two-electrode voltage clamp; Xenopus oocyte expression system; undergraduate biology laboratory;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
12
Recensione:
Indirizzi per estratti:
Indirizzo: Silver, WL Wake Forest Univ, Dept Biol, Winston Salem, NC 27106 USA Wake Forest Univ Winston Salem NC USA 27106 alem, NC 27106 USA
Citazione:
W.L. Silver e C.L. Browne, "Integration of laboratory exercises in developmental biology and neurobiology courses using the Xenopus oocyte expression system", J IND MIC B, 24(5), 2000, pp. 353-358

Abstract

This collaborative laboratory exercise integrates two upper division laboratory courses (Developmental Biology and Neurobiology) offered to biology majors at Wake forest University. The laboratory exercise involves the use of the Xenopus oocyte expression system to study the function of specific membrane receptors and ligand-activated channels. cDNA or mRNA for receptor proteins is injected into Xenopus oocytes. The oocytes are assayed for expression of receptor proteins and two-electrode voltage clamping is done to determine whether the expressed proteins are functional in the oocyte system. This series of laboratory exercises is innovative in its interdisciplinaryand collaborative approach to undergraduate teaching, and in its use of sophisticated molecular biological and physiological techniques in the undergraduate teaching laboratory. Students learn first-hand how these techniqueshave been used to achieve a new level of understanding of both developmentand neurobiology.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/09/20 alle ore 12:18:59