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Titolo:
Immunolocalization and adenoviral vector-mediated manganese superoxide dismutase gene transfer to experimental oral tumors
Autore:
Lam, EWN; Hammad, HM; Zwacka, R; Darby, CJ; Baumgardner, KR; Davidson, BL; Oberley, TD; Engelhardt, JF; Oberley, LW;
Indirizzi:
Univ Iowa, Coll Med, Dept Internal Med, Iowa City, IA 52242 USA Univ IowaIowa City IA USA 52242 pt Internal Med, Iowa City, IA 52242 USA Univ Iowa, Coll Med, Dept Anat & Cell Biol, Iowa City, IA 52242 USA Univ Iowa Iowa City IA USA 52242 nat & Cell Biol, Iowa City, IA 52242 USA Jordan Univ Sci & Technol, Fac Dent, Irbid, Jordan Jordan Univ Sci & Technol Irbid Jordan Technol, Fac Dent, Irbid, Jordan Western Gen Hosp, MRC, Human Genet Unit, Edinburgh EH4 2XU, Midlothian, Scotland Western Gen Hosp Edinburgh Midlothian Scotland EH4 2XU dlothian, Scotland Univ Michigan, Dept Cariol Restorat Sci & Endodont, Ann Arbor, MI 48109 USA Univ Michigan Ann Arbor MI USA 48109 & Endodont, Ann Arbor, MI 48109 USA William S Middleton Mem Vet Hosp, Madison, WI USA William S Middleton Mem Vet Hosp Madison WI USA et Hosp, Madison, WI USA Univ Iowa, Coll Med, Dept Radiol, Iowa City, IA 52242 USA Univ Iowa Iowa City IA USA 52242 ed, Dept Radiol, Iowa City, IA 52242 USA
Titolo Testata:
JOURNAL OF DENTAL RESEARCH
fascicolo: 6, volume: 79, anno: 2000,
pagine: 1410 - 1417
SICI:
0022-0345(200006)79:6<1410:IAAVMS>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
SMOOTH-MUSCLE CELLS; SYRIAN-HAMSTER TISSUES; IMMUNOHISTOCHEMICAL LOCALIZATION; GLUTATHIONE-PEROXIDASE; MALIGNANT PHENOTYPE; ANTIOXIDANT ENZYMES; KIDNEY DEVELOPMENT; EPITHELIAL-CELLS; PROSTATE-CANCER; NECROSIS FACTOR;
Keywords:
hamster cheek pouch; oral cancer; manganese superoxide dismutase; adenoviral gene transfer;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
60
Recensione:
Indirizzi per estratti:
Indirizzo: Lam, EWN Univ Alberta, Dept Dent, Dent Pharm Ctr 2085, Edmonton, AB T6G 2N8, Canada Univ Alberta Edmonton AB Canada T6G 2N8 nton, AB T6G 2N8, Canada
Citazione:
E.W.N. Lam et al., "Immunolocalization and adenoviral vector-mediated manganese superoxide dismutase gene transfer to experimental oral tumors", J DENT RES, 79(6), 2000, pp. 1410-1417

Abstract

The anti-oxidant enzyme system protects cellular macromolecules against damage from reactive oxygen species. One component of this system, manganese superoxide dismutase (MnSOD), has also been shown to display tumor suppressor gene-like activity. The purpose of this study was to examine changes in MnSOD expression during hamster cheek pouch carcinogenesis, and the effectsof MnSOD overexpression using an adenoviral vector. Tumor induction was carried out using 7,12-dimethylbenz[alpha] anthracene. Animals were killed atperiodic intervals, and cheek pouch tissues were excised and examined for MnSOD expression by immunohistochemistry and digital image analysis. We observed a reduction in MnSOD expression as early as 2 weeks after the start of carcinogen application. Low MnSOD expression persisted until the end of the 23-week experimental period. Solid hamster cheek pouch carcinoma xenografts were then established in nude mice. An adenoviral vector encoding the human MnSOD gene was delivered to the xenografts by direct injection. We observed high, immediate expression of MnSOD in the xenografts that persisted for 10 days following cessation of viral construct delivery. Delivery of the MnSOD construct resulted in a maximal 50% reduction in tumor growth compared with untreated controls. Our results suggest that MnSOD may be a tumor suppressor gene in the hamster cheek pouch model system.

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Documento generato il 02/07/20 alle ore 22:19:45