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Titolo:
Simultaneous high-performance liquid chromatographic determination of the antiretroviral agents amprenavir, nelfinavir, ritonavir, saquinavir, delavirdine and efavirenz in human plasma
Autore:
Proust, V; Toth, K; Hulin, A; Taburet, AM; Gimenez, F; Singlas, E;
Indirizzi:
Hop Necker Enfants Malad, Serv Pharm, F-75743 Paris 15, France Hop Necker Enfants Malad Paris France 15 Pharm, F-75743 Paris 15, France Hop Bicetre, Serv Pharm, F-94270 Le Kremlin Bicetre, France Hop Bicetre Le Kremlin Bicetre France F-94270 Le Kremlin Bicetre, France
Titolo Testata:
JOURNAL OF CHROMATOGRAPHY B
fascicolo: 2, volume: 742, anno: 2000,
pagine: 453 - 458
SICI:
1387-2273(20000609)742:2<453:SHLCDO>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
REVERSE-TRANSCRIPTASE INHIBITOR; ULTRAVIOLET DETECTION; PROTEASE INHIBITORS; CEREBROSPINAL-FLUID; QUANTIFICATION; SPECTROMETRY; SALIVA;
Keywords:
amprenavir; nelfinavir; ritonavir; saquinavir; delavirdine; efavirenz;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
16
Recensione:
Indirizzi per estratti:
Indirizzo: Gimenez, F Hop Necker Enfants Malad, Serv Pharm, 149 Rue Sevres, F-75743 Paris 15, France Hop Necker Enfants Malad 149 Rue Sevres Paris France 15 France
Citazione:
V. Proust et al., "Simultaneous high-performance liquid chromatographic determination of the antiretroviral agents amprenavir, nelfinavir, ritonavir, saquinavir, delavirdine and efavirenz in human plasma", J CHROMAT B, 742(2), 2000, pp. 453-458

Abstract

This article describes a method for the simultaneous determination of fourlicensed HIV protease inhibitors (amprenavir, nelfinavir, saquinavir and ritonavir) and two novel non-nucleoside reverse transcriptase inhibitors (efavirenz and delavirdine) in human plasma in a single run. Plasma samples (500 mu l) were treated by liquid-liquid extraction with methyl tert.-butyl ether. The compounds were separated by reversed-phase liquid chromatography on a C-18 column with spectrophotometric detection at 260 nm. The method islinear over the specific ranges investigated, accurate (inaccuracy <11.7%)and showed intra-day and inter-day precision within the ranges of 0.9-7.0 and 1.9-8.8%, respectively. The six compounds were stable in plasma after 6months of storage at -20 degrees C and after five freeze-thaw cycles. (C) 2000 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/01/20 alle ore 03:31:21