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Titolo:
In vivo protein-DNA interactions at the kinin B-1 receptor gene promoter: No modification on interleukin-1 beta or lipopolysaccharide induction
Autore:
Angers, M; Drouin, R; Bachvarova, M; Paradis, I; Marceau, F; Bachvarov, DR;
Indirizzi:
CHU Quebec, Hotel Dieu Quebec, Ctr Rech, Quebec City, PQ G1R 2J6, Canada CHU Quebec Quebec City PQ Canada G1R 2J6 Quebec City, PQ G1R 2J6, Canada Univ Laval, Fac Med, Dept Med Biol, Div Pathol, Quebec City, PQ G1K 7P4, Canada Univ Laval Quebec City PQ Canada G1K 7P4 Quebec City, PQ G1K 7P4, Canada Univ Laval, Fac Med, Dept Med, Quebec City, PQ G1K 7P4, Canada Univ LavalQuebec City PQ Canada G1K 7P4 Quebec City, PQ G1K 7P4, Canada CHU Quebec, Hop St Francois dAssise, Res Ctr, Unite Rech Genet Humaine & Mol, Quebec City, PQ, Canada CHU Quebec Quebec City PQ Canada Humaine & Mol, Quebec City, PQ, Canada
Titolo Testata:
JOURNAL OF CELLULAR BIOCHEMISTRY
fascicolo: 2, volume: 78, anno: 2000,
pagine: 278 - 296
SICI:
0730-2312(200005)78:2<278:IVPIAT>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
ISOLATED UMBILICAL ARTERY; NF-KAPPA-B; TRANSCRIPTION FACTORS; BRADYKININ B-1; CHROMOSOMAL DNA; UP-REGULATION; BINDING-SITE; EXPRESSION; ENHANCER; INVIVO;
Keywords:
G protein-coupled receptor; transient transfection; chloramphenicol acetyl transferase reporter gene; transcription factor binding motif; in vivo genomic footprinting; ligation-mediated PCR; primary cultures of human smooth muscle cells;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
50
Recensione:
Indirizzi per estratti:
Indirizzo: Bachvarov, DR CHU Quebec, Hotel Dieu Quebec, Ctr Rech, 11 Cote Palais, Quebec City, PQ G1R 2J6, Canada CHU Quebec 11 Cote Palais Quebec City PQ Canada G1R 2J6 nada
Citazione:
M. Angers et al., "In vivo protein-DNA interactions at the kinin B-1 receptor gene promoter: No modification on interleukin-1 beta or lipopolysaccharide induction", J CELL BIOC, 78(2), 2000, pp. 278-296

Abstract

The kinin B-1 receptor (B1R) gene is strongly upregulated following tissueinjury and inflammation. In an attempt to define the regulatory elements that account for the control of B1R gene expression, we have conducted in vivo footprinting analysis of the B1R gene promoter region In three human cell types. embryonic lung fibroblast cells (IMR-90), embryonic kidney cells (HEK-293), and primary cultures of vascular umbilical smooth muscle cells. Initial in vitro delineation of the B1R gene promoter by transient transfection experiments with a reporter gene indicated that a 1.4-kb region, located just upstream of the transcription Initiation site, bears ail the characteristics of a core promoter with a functional TATA box and additional positive and negative control elements, as some of them could be tissue-specific. In vivo ultraviolet and dimethylsulfate footprinting analyses of the 1.4-kb region revealed no difference between the footprint patterns in the th ree cell types stud led. We top nd that even in the noninduced state, the B1R gene promoter Is possibly bound by several sequence-specific DNA binding proteins (GATA-1, PEA3, AP-1, CAAT, Spl, Pit-1a, Oct-1. CREB). Some other footprints were detected on sequences that do not correspond to any known transcription factor binding site. No additional changes in protein-DNA complexes were observed upon treatment with interleukin-l beta (IL-1 beta) or bacterial lipopolysaccharide, shown previously to induce B1R gene expression. These results indicate that complex protein-DNA interactions exist at the B1R gene promoter prior to induction by external stimuli even in cells (HEK-293) that do not express a functional B1R. (C) 2000 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/09/20 alle ore 07:06:04