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Titolo:
Fluorescent differential display identifies circadian clock-regulated genes in Arabidopsis thaliana
Autore:
Kreps, JA; Muramatsu, T; Furuya, M; Kay, SA;
Indirizzi:
Scripps Res Inst, Dept Cell Biol, La Jolla, CA 92037 USA Scripps Res InstLa Jolla CA USA 92037 Cell Biol, La Jolla, CA 92037 USA Hitachi Adv Res Lab, Hatoyama, Saitama 3500395, Japan Hitachi Adv Res LabHatoyama Saitama Japan 3500395 Saitama 3500395, Japan
Titolo Testata:
JOURNAL OF BIOLOGICAL RHYTHMS
fascicolo: 3, volume: 15, anno: 2000,
pagine: 208 - 217
SICI:
0748-7304(200006)15:3<208:FDDICC>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
RNA-BINDING PROTEINS; CARBONIC-ANHYDRASE; MESSENGER-RNA; CHLAMYDOMONAS-REINHARDTII; TRANSGENIC PLANTS; MOLECULAR-CLONING; 2 CDNAS; EXPRESSION; LIGHT; TRANSCRIPTION;
Keywords:
circadian clock-controlled gene expression; fluorescent differential display; Arabidopsis thaliana;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
44
Recensione:
Indirizzi per estratti:
Indirizzo: Kay, SA Scripps Res Inst, Dept Cell Biol, La Jolla, CA 92037 USA Scripps Res Inst La Jolla CA USA 92037 ol, La Jolla, CA 92037 USA
Citazione:
J.A. Kreps et al., "Fluorescent differential display identifies circadian clock-regulated genes in Arabidopsis thaliana", J BIOL RHYT, 15(3), 2000, pp. 208-217

Abstract

Circadian rhythms in gene expression were first observed in plants more than 13 years ago, but the underlying mechanism controlling rhythmic gene expression is still not understood. The isolation of novel circadian clock-controlled genes (ccgs) is likely to provide new tools for studying circadian rhythms. Fluorescent differential display (FDD) was used to screen Arabidopsis thaliana mRNAs for cycling transcripts. Seventy PCR primer pairs were screened, and 17 different cycling bands were observed out of an estimated 10,500 bands screened. The identities of 10 bands were determined, and the rhythmic gene expression was confirmed using northern blot analysis. The 10 cycling bands represent 7 different genes, 6 of which are present in the databases and 1 that does not match anything in current databases. The rhythmic expression of the 7 genes is composed of four distinct phases of clock regulation. The results demonstrate that FDD can be used to isolate ccgs. The genes identified in this screen range from known A. thaliana ccgs, as well as genes shown to be clock controlled in other plant species, to a novel gene that may encode a pioneer protein. Further study of these ccgs is likely to increase our understanding of circadian-regulated gene expression.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 01:37:15