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Titolo:
Spectroscopic investigation of the molecular state of nystatin encapsulated in liposomes
Autore:
Moribe, K; Maruyama, K; Iwatsuru, M;
Indirizzi:
Teikyo Univ, Fac Pharmaceut Sci, Kanagawa 1990185, Japan Teikyo Univ Kanagawa Japan 1990185 armaceut Sci, Kanagawa 1990185, Japan
Titolo Testata:
INTERNATIONAL JOURNAL OF PHARMACEUTICS
fascicolo: 1, volume: 201, anno: 2000,
pagine: 37 - 49
SICI:
0378-5173(20000515)201:1<37:SIOTMS>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYETHYLENE-GLYCOL DERIVATIVES; AMPHOTERICIN-B; POLYENE ANTIBIOTICS; DILAUROYLPHOSPHATIDYLCHOLINE BILAYERS; PULMONARY ASPERGILLOSIS; SELF-ASSOCIATION; FLUORESCENCE; CHOLESTEROL; MEMBRANES; MICE;
Keywords:
nystatin; liposome; polyethylene glycol; fluorescence; circular dichroism spectra;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Maruyama, K Teikyo Univ, Fac Pharmaceut Sci, Kanagawa 1990185, Japan Teikyo Univ Kanagawa Japan 1990185 , Kanagawa 1990185, Japan
Citazione:
K. Moribe et al., "Spectroscopic investigation of the molecular state of nystatin encapsulated in liposomes", INT J PHARM, 201(1), 2000, pp. 37-49

Abstract

The stability and spectral properties of nystatin-encapsulating liposomes,composed of various combinations of dipalmitoyl phosphatidylcholine (DPPC), cholesterol (CH) and distearoyl-N-(monomethoxy poly(ethylene glycol)succinyl) phosphatidylethanolamine (DSPE-PEG), were studied in order to elucidate the molecular state and localization of nystatin encapsulated in liposomes. Localization of nystatin at the surface region of the liposomal membranewas investigated by PEG/dextran two-phase partition and measurement of thefluorescence quenching of nystatin by p-xylene-bis-pyridinium bromide (DPX). In DPPC/DSPE-PEG liposomes and DPPC/CH/DSPE-PEG liposomes, containing 151 and 160 mu g nystatin per mg lipid, respectively, nystatin appeared to bepresent at the surface region of the liposomal membranes. Self-quenching of nystatin fluorescence was observed in DPPC/CH and DPPC/CH/DSPP-PEG liposomes even at low encapsulated amounts, suggesting the localization of nystatin in CH-incorporating membranes. In CH-free liposomes, nystatin molecules were at first delocalized in the membranes and then self-associated at a higher level of encapsulation. Absorption and circular dichroism (CD) spectrawere also measured to examine the monomeric and aggregated states of nystatin in liposomes. High encapsulation efficacy was observed in DPPC and DPPC/DSPE-PEG liposomes, but the highest stability and retention of nystatin inliposomes were observed in DPPC/CH/DSPE-PEG liposomes, evaluated in terms of the nystatin and calcein release from nystatin-encapsulating liposomes in vitro. From the results, possible encapsulation mechanisms of nystatin inliposomes narrowed down to the following three points; interaction with lipid membrane, adsorption on the liposomal surface and complex formation with DSPE-PEG. (C) 2000 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/09/20 alle ore 13:05:41