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Titolo:
The putative proteinase maturation protein A of Streptococcus pneumoniae is a conserved surface protein with potential to elicit protective immune responses
Autore:
Overweg, K; Kerr, A; Sluijter, M; Jackson, MH; Mitchell, TJ; de Jong, APJM; de Groot, R; Hermans, PWM;
Indirizzi:
Erasmus Univ, Sophia Childrens Hosp, Dept Pediat, Rotterdam, Netherlands Erasmus Univ Rotterdam Netherlands Dept Pediat, Rotterdam, Netherlands Natl Inst Publ Hlth & Environm, Lab Organ Analyt Chem, NL-3720 BA Bilthoven, Netherlands Natl Inst Publ Hlth & Environm Bilthoven Netherlands NL-3720 BA herlands Univ Glasgow, Div Infect & Immun, Glasgow, Lanark, Scotland Univ Glasgow Glasgow Lanark Scotland & Immun, Glasgow, Lanark, Scotland Natl Inst Biol Stand & Controls, Cell Biol & Imaging Sect, Potters Bar, Herts, England Natl Inst Biol Stand & Controls Potters Bar Herts England Herts, England
Titolo Testata:
INFECTION AND IMMUNITY
fascicolo: 7, volume: 68, anno: 2000,
pagine: 4180 - 4188
SICI:
0019-9567(200007)68:7<4180:TPPMPA>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
NASOPHARYNGEAL COLONIZATION; PNEUMOCOCCAL CHALLENGE; RESISTANT PNEUMOCOCCI; BINDING PROTEIN; PHASE VARIATION; PSPA; MICE; VIRULENCE; PNEUMOLYSIN; TRANSFORMATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
55
Recensione:
Indirizzi per estratti:
Indirizzo: Hermans, PWM Erasmus Univ, Pediat Lab, Room Ee 1500,POB 1738, NL-3000 DR Rotterdam, Netherlands Erasmus Univ Room Ee 1500,POB 1738 Rotterdam Netherlands NL-3000 DR
Citazione:
K. Overweg et al., "The putative proteinase maturation protein A of Streptococcus pneumoniae is a conserved surface protein with potential to elicit protective immune responses", INFEC IMMUN, 68(7), 2000, pp. 4180-4188

Abstract

Surface-exposed proteins often play an important role in the interaction between pathogenic bacteria and their host. We isolated a pool of hydrophobic, surface associated proteins of Streptococus pneumoniae. The opsonophagocytic activity of hyperimmune serum raised against this protein fraction washigh and species specific, Moreover, the opsonophagocytic activity was independent of the capsular type and chromosomal genotype of the pneumococcus. Since the opsonophagocytic activity is presumed to correlate with in vivo protection, these data indicate that the protein fraction has the potentialto elicit species-specific immune protection with cross-protection againstvarious pneumococcal strains. Individual proteins in the extract were purified by two-dimensional gel electrophoresis. Antibodies raised against three distinct proteins contributed to the opsonophagocytic activity of the serum, The proteins were identified by mass spectrometry and N-terminal amino acid sequencing. Two proteins were the previously characterized pneumococcal surface protein A and oligopeptide-binding lipoprotein AmiA, The third protein was the recently identified putative proteinase maturation protein A (PpmA), which showed homology to members of the family of peptidyl-prolyl cis/trans isomerases, Immunoelectron microscopy demonstrated that PpmA was associated with the pneumococcal surface. In addition, PpmA was shown to elicit species-specific opsonophagocytic antibodies that were crossreactive with various pneumococcal strains. This antibody cross-reactivity was in linewith the limited sequence variation of ppmA. The importance of PpmA in pneumococcal pathogenesis was demonstrated in a mouse pneumonia model. Pneumococcal ppmA-deficient mutants showed reduced virulence. The properties of PpmA reported here indicate its potential for inclusion in multicomponent protein vaccines.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/10/20 alle ore 11:47:56