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Titolo:
Comparative anatomy of the cerebellar cortex in mice lacking vimentin, GFAP, and both vimentin and GFAP
Autore:
Ribotta, MGY; Langa, F; Menet, V; Privat, A;
Indirizzi:
Univ Montpellier 2, INSERM, U336, F-34095 Montpellier 05, France Univ Montpellier 2 Montpellier France 05 F-34095 Montpellier 05, France Inst Pasteur, Dev Biol Lab, Paris, France Inst Pasteur Paris FranceInst Pasteur, Dev Biol Lab, Paris, France
Titolo Testata:
GLIA
fascicolo: 1, volume: 31, anno: 2000,
pagine: 69 - 83
SICI:
0894-1491(200007)31:1<69:CAOTCC>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
FIBRILLARY ACIDIC PROTEIN; INTERMEDIATE FILAMENT PROTEIN; HUMAN GLIOMA-CELLS; REACTIVE ASTROCYTES; MOTOR COORDINATION; SIZED FILAMENTS; HEAD DOMAIN; MUTANT MICE; RAT EMBRYO; BRAIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
49
Recensione:
Indirizzi per estratti:
Indirizzo: Privat, A Univ Montpellier 2, INSERM, U336, BP 106,Pl E Bataillon, F-34095Montpellier 05, France Univ Montpellier 2 BP 106,Pl E Bataillon Montpellier France 05
Citazione:
M.G.Y. Ribotta et al., "Comparative anatomy of the cerebellar cortex in mice lacking vimentin, GFAP, and both vimentin and GFAP", GLIA, 31(1), 2000, pp. 69-83

Abstract

In the cerebellum of adult mammals, glial fibrillary acidic protein (GFAP)and vimentin (VIM) are coexpressed in Golgi epithelial cells (GEC), also known as Bergmann glia. In this study we used three transgenic knockout mice(GFAP, VIM and double GFAP and VIM) to analyze the involvement of these proteins in the building of glial filaments and in neuron-glia interactions. The cerebella of VIM, GFAP, and GFAP/VIM mutant mice were processed by the rapid Golgi method and also for electron microscopy. In VIM mutant mice, Bergmann fibers are hypertrophic with thickened appendages. In the electron microscope they appear as large glial profiles devoid of glial filaments, with embedded dendritic thorns and parallel fiber boutons. In addition, signsof degeneration are observed in Purkinje cells. In GFAP mutant mice, GEC exhibit fine, delicate processes, as those seen in wild-type animals, however, a large accumulation of lamellae and granular appendages was observed along their surfaces, which came into contact with each other. The electron microscope exhibited fine and scarce astroglial profiles containing some glial filaments, a stunted glia limitans, and the presence of large extracellular spaces. In double mutant mice, the two phenotypes are expressed but appear attenuated, with a total absence of glial filaments and the general appearance of immaturity for GEC. In conclusion, it appears that the absence of each of the proteins yields a specific phenotype and that the defects arenot necessarily additive. (C) 2000 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 16:20:29