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Titolo:
Tight junction properties of the immortalized human bronchial epithelial cell lines Calu-3 and 16HBE14o-
Autore:
Wan, H; Winton, HL; Soeller, C; Stewart, GA; Thompson, PJ; Gruenert, DC; Cannell, MB; Garrod, DR; Robinson, C;
Indirizzi:
St George Hosp, Sch Med, Dept Pharmacol & Clin Pharmacol, London SW17 0RE,England St George Hosp London England SW17 0RE harmacol, London SW17 0RE,England Univ Western Australia, Queen Elizabeth II Med Ctr, Dept Microbiol, Perth,WA 6009, Australia Univ Western Australia Perth WA Australia 6009 , Perth,WA 6009, Australia Univ Western Australia, Queen Elizabeth II Med Ctr, Dept Med, Perth, WA 6009, Australia Univ Western Australia Perth WA Australia 6009 Perth, WA 6009, Australia Univ Calif San Francisco, Gene Therapy Core Ctr, Dept Lab Med, San Francisco, CA 94143 USA Univ Calif San Francisco San Francisco CA USA 94143 ancisco, CA 94143 USA Univ Manchester, Sch Biol Sci, Manchester, Lancs, England Univ ManchesterManchester Lancs England Sci, Manchester, Lancs, England
Titolo Testata:
EUROPEAN RESPIRATORY JOURNAL
fascicolo: 6, volume: 15, anno: 2000,
pagine: 1058 - 1068
SICI:
0903-1936(200006)15:6<1058:TJPOTI>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
DUST MITE PROTEINASES; POSSIBLE INVOLVEMENT; OCCLUDING JUNCTIONS; GELATINASE ACTIVITY; PERMEABILITY; ZO-1; ADHESION; DOMAIN; AUGMENTATION; ACTIVATION;
Keywords:
airway epithelium; occludin; permeability; tight junction; zonulae adherentes; ZO-1;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Robinson, C St George Hosp, Sch Med, Dept Pharmacol & Clin Pharmacol, Cranmer Terrace,London SW17 0RE, England St George Hosp Cranmer Terrace LondonEngland SW17 0RE ngland
Citazione:
H. Wan et al., "Tight junction properties of the immortalized human bronchial epithelial cell lines Calu-3 and 16HBE14o-", EUR RESP J, 15(6), 2000, pp. 1058-1068

Abstract

Tight junctions (TJs) make a vital contribution to the barrier properties of the airway lining. Opening of TJs, or their frank cleavage, is suspectedas a pathophysiological event in the lung, but research into the cellular and molecular mechanisms involved has been impeded by technical limitationsof available experimental models. The authors have compared the propertiesof two epithelial cell lines derived from bronchial epithelium to explore whether these cell lines could constitute appropriate tools for the study of TJ regulation in bronchial epithelium. Investigations of TJs in 16HBE14o- cells and Calu-3 cells were made by fluorescent antibody labelling in conjunction with wide-field, confocal or 2-photon molecular excitation microscopy (2PMEM). The presence of TJ proteins was confirmed by immunoblotting and functional properties of the monolayerswere studied by measurements of transepithelial electrical resistance and mannitol permeability. Cells of both lines formed confluent monolayers In which the cells expressed the TJ proteins occludin and ZO-1 in continuous circumferential patternssuggestive of func tional TJs. This interpretation was supported by the development of transepithelial electrical resistances and of low paracellularpermeability to solutes. Within the limits of resolution offered by 2PMEM,occludin and ZO-1 appeared to colocalize at TJs. These studies suggest that the 16HBE14o- cells and Calu3 cell lines are potentially useful in vitro models to study how tight junction opening or cleavage changes the functional barrier properties of bronchial epithelium.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/11/20 alle ore 04:15:55