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Titolo:
Genotoxic effects of alpha-endosulfan and beta-endosulfan on human HepG2 cells
Autore:
Lu, YQ; Morimoto, K; Takeshita, T; Takeuchi, T; Saito, T;
Indirizzi:
Osaka Univ, Grad Sch Med, Dept Social & Environm Med F1, Suita, Osaka 5650871, Japan Osaka Univ Suita Osaka Japan 5650871 Med F1, Suita, Osaka 5650871, Japan Hokkaido Univ, Sch Med, Dept Prevent Med, Lab Environm Biol, Sapporo, Hokkaido 060, Japan Hokkaido Univ Sapporo Hokkaido Japan 060 ol, Sapporo, Hokkaido 060, Japan
Titolo Testata:
ENVIRONMENTAL HEALTH PERSPECTIVES
fascicolo: 6, volume: 108, anno: 2000,
pagine: 559 - 561
SICI:
0091-6765(200006)108:6<559:GEOAAB>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
BINDING GLOBULIN SECRETION; HUMAN HEPATOMA-CELLS; VITAMIN-C; ESTROGEN; PESTICIDES; CHEMICALS; SYSTEMS; HAMSTER; INVITRO;
Keywords:
alpha-endosulfan; beta-endosulfan; genotoxicity; HepG2; micronuclei; MN; single-cell gel electrophoresis; SCG; sister chromatid exchanges; SCE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
23
Recensione:
Indirizzi per estratti:
Indirizzo: Morimoto, K Osaka Univ, Grad Sch Med, Dept Social & Environm Med F1, 2-2 Yamadaoka, Suita, Osaka 5650871, Japan Osaka Univ 2-2 Yamadaoka Suita Osaka Japan 5650871 0871, Japan
Citazione:
Y.Q. Lu et al., "Genotoxic effects of alpha-endosulfan and beta-endosulfan on human HepG2 cells", ENVIR H PER, 108(6), 2000, pp. 559-561

Abstract

alpha-Endosulfan and beta-endosulfan are isomers of endosulfan, a pesticide used worldwide. In this study, we examined the genotoxicity of alpha- andbeta-endosulfan in vitro with a HepG2 cell line. We used sister chromatid exchanges (SCE), micronuclei (MN), and DNA strand breaks as detected by single-cell gel electrophoresis (SCG) assays as biomarkers to judge the genotoxicity of alpha- and beta-endosulfan at concentrations from 1 x 10(-12) M to 1 x 10(-3) M. After treating HepG2 cells for 48 hr with beta-endosulfan, SCE showed a significant increase at concentrations from 1 x 10(-7) M to 1 x 10(-5) M, and MN showed a significant increase at concentrations from 5 x10(-5) M to 1 x 10(-3) M. alpha-Endosulfan failed to show significant effects in both the SCE and MN assays. After treating HepG2 cells with alpha- or beta-endosulfan for 1 hr, DNA strand breaks were significantly induced byalpha-endosulfan at concentrations from 2 x 10(-4) M to 1 x 10-3 M, and bybeta-endosulfan at 1 x 10-3 M. The results of this study suggest that bothalpha- and beta-endosulfan are genotoxic to HepG2 cells and that the genotoxicity of beta-endosulfan seems stronger than that of alpha-endosulfan.

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Documento generato il 30/09/20 alle ore 23:03:13