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Titolo:
The bHLH class protein pMesogenin1 can specify paraxial mesoderm phenotypes
Autore:
Yoon, JK; Moon, RT; Wold, B;
Indirizzi:
CALTECH, Div Biol, Pasadena, CA 91125 USA CALTECH Pasadena CA USA 91125CALTECH, Div Biol, Pasadena, CA 91125 USA Univ Washington, Sch Med, Howard Hughes Med Inst, Dept Pharmacol, Seattle,WA 98195 USA Univ Washington Seattle WA USA 98195 Dept Pharmacol, Seattle,WA 98195 USA Univ Washington, Sch Med, Ctr Dev Biol, Seattle, WA 98195 USA Univ Washington Seattle WA USA 98195 Ctr Dev Biol, Seattle, WA 98195 USA
Titolo Testata:
DEVELOPMENTAL BIOLOGY
fascicolo: 2, volume: 222, anno: 2000,
pagine: 376 - 391
SICI:
0012-1606(20000615)222:2<376:TBCPPC>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
LOOP-HELIX PROTEIN; BOX TRANSCRIPTION FACTOR; XENOPUS-EMBRYOS; SOMITE FORMATION; LUNATIC FRINGE; DELTA-HOMOLOG; EARLY RESPONSE; NOTCH PATHWAY; GENE; EXPRESSION;
Keywords:
bHLH gene; MesP1; MesP2; Thylacine; paraxial mesoderm; somitomeres; somite; Xenopus laevis; mouse;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
67
Recensione:
Indirizzi per estratti:
Indirizzo: Wold, B CALTECH, Div Biol, Pasadena, CA 91125 USA CALTECH Pasadena CA USA91125 H, Div Biol, Pasadena, CA 91125 USA
Citazione:
J.K. Yoon et al., "The bHLH class protein pMesogenin1 can specify paraxial mesoderm phenotypes", DEVELOP BIO, 222(2), 2000, pp. 376-391

Abstract

A new bHLH gene from mouse that we call pMesogenin1 (referring to paraxialmesoderm-specific expression and regulatory capacities) and its candidate ortholog from Xenopus were isolated and studied comparatively, Tn both organisms the gene is specifically expressed in unsegmented paraxial mesoderm and its immediate progenitors. A striking feature of pMesogenin1 expression is that it terminates abruptly in presumptive somites (somitomeres). Somitomeres rostral to the pMesogenin1 domain strongly upregulate expression of pMesogenin's closest known paralogs, MesP1 and MesP2 (Thylacine1/2 in Xenopus), Subsequently, the most rostral somitomere becomes a new somite and expression of MesP1/2 is sharply downregulated before this transition. Thus, expression patterns of these bHLH genes, together with that of an additional bHLH gene in the mouse, Paraxis, collectively define discrete but highly dynamic prepatterned subdomains of the paraxial mesoderm, In functional assays, we show that pMesogenin1 from either mouse or frog can efficiently drivenonmesodermal cells to assume a phenotype with molecular and cellular characteristics of early paraxial mesoderm, Among genes induced by added pMesogenin1 is Xwnt-8, a signaling factor that induces a similar repertoire of marker genes and a similar cellular phenotype, Additional target genes Induced by pMesogenin1 are ESR4/5, regulators known to play a significant role insegmentation of paraxial mesoderm (W. C. Jen et al., 1999, Genes Dev. 13, 1486-1449). pMesogenin1 differs from other known mesoderm-inducing transcription factors because it does not also activate a dorsal (future axial) mesoderm phenotype, suggesting that pMesogenin1 is involved in specifying paraxial mesoderm. In the context of the intact hog embryo, ectopic pMesogenin1also actively suppressed axial mesoderm markers and disrupted normal formation of notochord. In addition, we found evidence for cross-regulatory interactions between pMesogenin1 and T-box transcription factors, a family of genes normally expressed in a broader pattern and known to induce multiple types of mesoderm. Based on our results and results from prior studies of related bHLH genes, we propose that pMesogenin1 and its closest known relatives, MesP1/2 (in mouse) and Thylacine1/2 (in Xenopus), comprise a bHLH subfamily devoted to formation and segmentation of paraxial mesoderm. (C) 2000 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 14/07/20 alle ore 19:40:00