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Titolo:
Targeting of cre to the Foxg1 (BF-1) locus mediates loxP recombination in the telencephalon and other developing head structures
Autore:
Hebert, JM; McConnell, SK;
Indirizzi:
Stanford Univ, Dept Biol Sci, Stanford, CA 94305 USA Stanford Univ Stanford CA USA 94305 Dept Biol Sci, Stanford, CA 94305 USA
Titolo Testata:
DEVELOPMENTAL BIOLOGY
fascicolo: 2, volume: 222, anno: 2000,
pagine: 296 - 306
SICI:
0012-1606(20000615)222:2<296:TOCTTF>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
CEREBRAL HEMISPHERES; CELL-MIGRATION; MOUSE EMBRYO; FOREBRAIN; EXPRESSION; NOTCH1; BRAIN; OTX2; NEUROECTODERM; SPECIFICATION;
Keywords:
BF-1; Foxg1; Cre/loxP; Foxg1-Cre; telencephalon; cerebral cortex; hippocampus; striatum; R26R; Z/AP;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
25
Recensione:
Indirizzi per estratti:
Indirizzo: Hebert, JM Stanford Univ, Dept Biol Sci, Stanford, CA 94305 USA Stanford Univ Stanford CA USA 94305 ci, Stanford, CA 94305 USA
Citazione:
J.M. Hebert e S.K. McConnell, "Targeting of cre to the Foxg1 (BF-1) locus mediates loxP recombination in the telencephalon and other developing head structures", DEVELOP BIO, 222(2), 2000, pp. 296-306

Abstract

The use of genetics to study the development of the telencephalon and derivatives such as the cerebral cortex has bern limited. The telencephalon begins to form midway through gestation, and targeted mutations in genes suspected of playing roles in its development often lead to early phenotypes that preclude analysis of their role at later stages. This problem can be circumvented using a Cre/loxP recombination system, A mouse line was produced in which cre was targeted to the Foxg1 (BF-1) locus, a gene expressed specifically in the telencephalon and discrete head structures, Crosses between Foxg1-Cre mice and three separate loxP reporter mice generated embryos with recombination patterns matching that expected from the normal pattern of Foxg1 expression, Recombination occurs invariably in the telencephalon, anterior optic vesicle, otic vesicle, facial and head ectoderm, olfactory epithelium, mid-hindbrain junction, and pharyngeal pouches. Recombination in someanimals also occurs less efficiently in tissues not known to express Foxg1. We show that the genetic background of the parental mice and the loxP target allele can each contribute to differences in the exact pattern of recombination. Collectively, these data show that Foxg1-Cre mice should be useful in the deletion or ectopic expression of any floxed target gene in a Foxg1-like pattern. (C) 2000 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/01/20 alle ore 17:19:52