Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Cellular signaling by the potent bronchoconstrictor endothelin-1 in airwaysmooth muscle
Autore:
Fehr, JJ; Hirshman, CA; Emala, CW;
Indirizzi:
Johns Hopkins Med Inst, Dept Anesthesiol, Baltimore, MD 21205 USA Johns Hopkins Med Inst Baltimore MD USA 21205 ol, Baltimore, MD 21205 USA Johns Hopkins Med Inst, Dept Environm Hlth Sci, Baltimore, MD 21205 USA Johns Hopkins Med Inst Baltimore MD USA 21205 ci, Baltimore, MD 21205 USA
Titolo Testata:
CRITICAL CARE MEDICINE
fascicolo: 6, volume: 28, anno: 2000,
pagine: 1884 - 1888
SICI:
0090-3493(200006)28:6<1884:CSBTPB>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
BRONCHOALVEOLAR LAVAGE FLUID; HUMAN RIGHT ATRIUM; VASOCONSTRICTOR PEPTIDE; PROSTAGLANDINS MEDIATE; RECEPTOR ANTAGONIST; ADENYLATE-CYCLASE; ISOLATED TRACHEA; CELLS; ASTHMA; CONTRACTION;
Keywords:
endothelin-1; endothelin receptors; porcine airway smooth muscle; inositol phosphate production; adenylyl cyclase; BQ-485; BQ-788;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
45
Recensione:
Indirizzi per estratti:
Indirizzo: Fehr, JJ Johns Hopkins Med Inst, Dept Anesthesiol, Baltimore, MD 21205 USAJohns Hopkins Med Inst Baltimore MD USA 21205 more, MD 21205 USA
Citazione:
J.J. Fehr et al., "Cellular signaling by the potent bronchoconstrictor endothelin-1 in airwaysmooth muscle", CRIT CARE M, 28(6), 2000, pp. 1884-1888

Abstract

Objective: The objective of this study was to determine whether the potentbronchoconstrictor endothelin-l was coupled to the activation of the inositol phosphate and/or inhibition of the cyclic adenine monophosphate second messenger pathways in porcine airway smooth muscle. Design: Prospective, controlled, in vitro, nonblinded study. Setting: University biochemical and molecular biological research laboratory. Subjects: Pigs of both genders. Interventions: Airway smooth muscle was dissected from the trachea of pigsexsanguinated under anesthesia. Airway smooth muscle from six animals preloaded with H-3-myoinositol was exposed to endothelin-l, carbachol (positivecontrol) or vehicle for 30 mins. Some tissues were pretreated with antagonists selective for the ETA (BQ-485) and ETB (BQ-788) endothelin receptor subtypes. Newly synthesized H-3-inositol phosphates were recovered by column chromatography. Airway smooth muscle from an additional 7 pigs was homogenized and incubated in the presence of P-32-alpha-adenosine triphosphate, guanosine triphosphate (GTP) and either carbachol or endothelin to measure theinhibitory influence of carbachol (positive control) or endothelin on GTP-stimulated adenylyl cyclase activity. Newly synthesized P-32-cyclic adenosine monophosphate was isolated by sequential column chromatography over Dowex and alumina,Measurements and Main Results: Total inositol phosphates increased in porcine airway smooth muscle in response to either carbachol or endothelin. Theendothelin receptor antagonist BQ-485 (ETA selective) but not BQ-788 (ETB selective) dose-dependently inhibited endothelin-l induced inositol phosphate accumulation, In adenylyl cyclase assays, carbachol (positive control), but not endothlein-1, significantly inhibited GTP-stimulated adenylyl cyclase activity,Conclusion: Endothelin-1 couples to the activation of the inositol phosphate pathway via the ETA receptor subtype but does not couple to inhibition of the adenylyl cyclase pathway in porcine airway smooth muscle. The potent bronchoconstrictive effects of endothelin likely involve the acute activation of the inositol phosphate pathway in airway smooth muscle.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/04/20 alle ore 02:14:17