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Titolo:
Stroma-dependent maintenance of cytokine responsive hematopoietic progenitor cells derived from long-term bone marrow culture
Autore:
Okubo, T; Matsui, N; Yanai, N; Obinata, M;
Indirizzi:
Tohoku Univ, Inst Dev Aging & Canc, Dept Cell Biol, Aoba Ku, Sendai, Miyagi 9808575, Japan Tohoku Univ Sendai Miyagi Japan 9808575 Ku, Sendai, Miyagi 9808575, Japan
Titolo Testata:
CELL STRUCTURE AND FUNCTION
fascicolo: 2, volume: 25, anno: 2000,
pagine: 133 - 139
SICI:
0386-7196(200004)25:2<133:SMOCRH>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
STEM-CELLS; SELF-RENEWAL; IN-VITRO; DIFFERENTIATION; PROLIFERATION; LINE; STIMULATION;
Keywords:
stromal cell; cobblestone; hematopoietic progenitor;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
26
Recensione:
Indirizzi per estratti:
Indirizzo: Yanai, N Tohoku Univ, Inst Dev Aging & Canc, Dept Cell Biol, Aoba Ku, Sendai, Miyagi 9808575, Japan Tohoku Univ Sendai Miyagi Japan 9808575 i, Miyagi 9808575, Japan
Citazione:
T. Okubo et al., "Stroma-dependent maintenance of cytokine responsive hematopoietic progenitor cells derived from long-term bone marrow culture", CELL STRUCT, 25(2), 2000, pp. 133-139

Abstract

Hematopoietic cells maintained for long periods on primary cultures of bone marrow stromal cells formed cobblestone colonies (Dexter's long-term bonemarrow culture, LTBC), These stably maintained hematopoietic cells (for 4 months) were transferred to a coculture on an established spleen stromal cell Line (MSS62), and maintained under stromal cell layer, where they retained their invasive ability in the restricted space between the stromal cell layer and culture substratum (DFC culture). DFC contained lineage-negative (Lin(-)), c-Kit(+), Sca-1(+) cells and spontaneously produced Mac-1(+), Gr-1(+) cells, DFC could not grow in the absence of MSS62 stromal cells, although, GM-CSF, IL-3, or IL-7 stimulated its growth, Production of granulocyteand monocytic cells was maintained by CM-CSP or IL-3 while it was decreased by IL-7, RT-PCR analysis showed that the IL-7 responsive cell population expressed early lymphoid markers (Ikaros, Pax-5, Oct-2, Rag-1, TdT, IL-7R and I mu), while lacking expression of receptors for G-CSF (G-CSFR) and for ICI-CSF (M-CSFR), or myeloperoxidase (MPO). These results suggested that DFC simultaneously contained lymphoid-committed progenitors and myeloid-committed progenitors, and that cytokines may expand their responding progenitorcells under the influence of signals provided by the stromal cells. Such astromal cell-dependent culture system may be useful to analyze the switching mechanism from constitutive to inducible hematopoiesis in vitro.

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Documento generato il 04/12/20 alle ore 09:25:09