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Titolo:
Potassium depolarization-induced cAMP stimulates somatostatin mRNA levels in cultured diencephalic neurons
Autore:
Tolon, R; Franco, FS; Villuendas, G; Vicente, AB; Palacios, N; Cacicedo, L;
Indirizzi:
Hosp Ramon y Cajal, Endocrine Serv, E-28034 Madrid, Spain Hosp Ramon y Cajal Madrid Spain E-28034 rine Serv, E-28034 Madrid, Spain Hosp Carlos III, Madrid 28034, Spain Hosp Carlos III Madrid Spain 28034Hosp Carlos III, Madrid 28034, Spain Inst Salud Carlos III, Madrid 28034, Spain Inst Salud Carlos III Madrid Spain 28034 Carlos III, Madrid 28034, Spain
Titolo Testata:
BRAIN RESEARCH
fascicolo: 2, volume: 868, anno: 2000,
pagine: 338 - 346
SICI:
0006-8993(20000623)868:2<338:PDCSSM>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
C-FOS TRANSCRIPTION; GENE-EXPRESSION; ADENYLYL-CYCLASE; CEREBROCORTICAL CELLS; CALCIUM REGULATION; PROTEIN-KINASE; FACTOR CREB; CA2+; ELEMENT; IDENTIFICATION;
Keywords:
somatostatin; depolarization; potassium; cAMP; PKA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Cacicedo, L Hosp Ramon y Cajal, Endocrine Serv, Carretera Comenar Km 9, E-28034 Madrid, Spain Hosp Ramon y Cajal Carretera Comenar Km 9 Madrid SpainE-28034
Citazione:
R. Tolon et al., "Potassium depolarization-induced cAMP stimulates somatostatin mRNA levels in cultured diencephalic neurons", BRAIN RES, 868(2), 2000, pp. 338-346

Abstract

In the nervous system, signals transmitted across synapses are known to regulate gene expression in the postsynaptic cells. This process often involves membrane depolarization and subsequent elevation of intracellular Ca2+. We have previously demonstrated in fetal cerebrocortical cells, that somatostatin (SS) mRNA levels can be induced by depolarizing agents such as high potassium concentrations and veratridine (VTD), and that these effects are calcium dependent. SS expression is regulated by cAMP, and in the cerebral cortex adenylate cyclase activity is regulated through fluctuations in intracellular Ca2+ concentrations. The present experiments were undertaken to determine the mechanism by which calcium upregulates the levels of SS mRNA. Cerebrocortical cells from 17-day-old fetuses were exposed to the differentagents for 24 h and the levels of SS mRNA were measured by Northern blot. Incubation of cells with the calcium channel antagonist nifedipine (Nf), the calcium chelating agent EGTA, calcium free KRB and the calcium calmodulininhibitors trifluoroperazine (TFP) and the napthelene sulfonamide, W7, resulted in the inhibition of K+-induced SS mRNA levels. K+-evoked depolarization increased the intracellular concentration of cAMP and this effect was antagonized by verapamil (VPM). Forskolin (Fk) provoked a higher increment in cAMP concentration than potassium, although the induction of SS mRNA was more evident following K+ depolarization indicating a lack of correlation between levels of cAMP and induction of SS mRNA. The role of K+-induced cAMPon the increment of SS mRNA that occurred upon membrane depolarization wasfurther explored with the inhibitor of protein kinase A (PKA), Rp cAMP whose presence significantly reduced depolarization-induced SS mRNA levels. This study confirms that Ca2+ influx is required for K+ depolarization-induced stimulation of cAMP whereby the increment of SS mRNA is partly produced. (C) 2000 Elsevier Science B.V. All rights reserved.

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Documento generato il 30/11/20 alle ore 07:03:23