Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Abasic DNA structure, reactivity, and recognition
Autore:
Lhomme, J; Constant, JF; Demeunynck, M;
Indirizzi:
Univ Grenoble 1, UMR CNRS 5616, LEDSS, F-38041 Grenoble 9, France Univ Grenoble 1 Grenoble France 9 616, LEDSS, F-38041 Grenoble 9, France
Titolo Testata:
BIOPOLYMERS
fascicolo: 2, volume: 52, anno: 1999,
pagine: 65 - 83
SICI:
0006-3525(1999)52:2<65:ADSRAR>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
APURINIC APYRIMIDINIC SITES; BASE-EXCISION-REPAIR; NUCLEAR-MAGNETIC-RESONANCE; MOLECULAR MECHANICS CALCULATIONS; EFFICIENT CHEMICAL SYNTHESIS; REFINED SOLUTION STRUCTURE; TOPOISOMERASE-II POISONS; SINGLE-STRANDED-DNA; UV ENDONUCLEASE-V; MAMMALIAN-CELLS;
Keywords:
DNA; abasic; apurinic; apyrimidinic; oligonucleotide synthesis; reactivity; structure; enzymology; cleaving agent; repair inhibitors;
Tipo documento:
Review
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
155
Recensione:
Indirizzi per estratti:
Indirizzo: Lhomme, J Univ Grenoble 1, UMR CNRS 5616, LEDSS, BP 53, F-38041 Grenoble 9, France Univ Grenoble 1 BP 53 Grenoble France 9 8041 Grenoble 9, France
Citazione:
J. Lhomme et al., "Abasic DNA structure, reactivity, and recognition", BIOPOLYMERS, 52(2), 1999, pp. 65-83

Abstract

Loss of a base in DNA, i.e., creation of an abasic site leaving a deoxyribose residue in the strand, is a frequent lesion that may occur spontaneously, or under the action of radiations and alkylating agents, or enzymatically as an intermediate in the repair of modified or abnormal bases. The abasic site lesion is mutagenic or lethal if not repaired From a chemical point of view,the abasic site is an alkali-labile residue that leads to strand breakage through beta- and delta- elimination Progress in the understanding of the chemistry and enzymology of abasic DNA largely relies upon the study of synthetic abasic duplexes. Several efficient synthetic methods have thusbeen developed to introduce the lesion (or a stable analogue) at defined position in the sequence. Physicochemical and spectroscopic examination of such duplexes, including calorimetry, melting temperature, high-field nmr and molecular modeling indicate that the lesion strongly destabilizes the duplex, although remaining in the canonical B-form with structural modifications strictly located at the site of the lesion. Probes have been developed to titrate the damage in DNA in vitro. Series of molecules have been devisedto recognize specifically the abasic site, exhibiting a cleavage activity and mimicking the AP nucleases. Others have been prepared that bind strongly to the abasic site and show promise in potentiating the cytotoxic and antitumor activity of the clinically used nitrosourea (bis-chloroethylnitrosurea). (C) 2000 John Wiley & Sons, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 14:09:09