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Titolo:
Solution structure of an RNA duplex including a C-U base pair
Autore:
Tanaka, Y; Kojima, C; Yamazaki, T; Kodama, TS; Yasuno, K; Miyashita, S; Ono, A; Ono, A; Kainosho, M; Kyogoku, Y;
Indirizzi:
Osaka Univ, Inst Prot Res, Div Mol Biophys, Suita, Osaka 5650871, Japan Osaka Univ Suita Osaka Japan 5650871 Biophys, Suita, Osaka 5650871, Japan Tokyo Metropolitan Univ, Fac Sci, Tokyo 1920364, Japan Tokyo Metropolitan Univ Tokyo Japan 1920364 ac Sci, Tokyo 1920364, Japan
Titolo Testata:
BIOCHEMISTRY
fascicolo: 24, volume: 39, anno: 2000,
pagine: 7074 - 7080
SICI:
0006-2960(20000620)39:24<7074:SSOARD>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
N-15 NMR; NITROGEN-15-LABELED OLIGODEOXYNUCLEOTIDES; DOUBLE HELIX; HAIRPIN RIBOZYMES; CRYSTAL-STRUCTURE; RIBOSOMAL-RNA; SPECTROSCOPY; MISMATCHES; DYNAMICS; LOOP;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
37
Recensione:
Indirizzi per estratti:
Indirizzo: Kyogoku, Y Osaka Univ, Inst Prot Res, Div Mol Biophys, 3-2 Yamadaoka, Suita, Osaka 5650871, Japan Osaka Univ 3-2 Yamadaoka Suita Osaka Japan 5650871 50871, Japan
Citazione:
Y. Tanaka et al., "Solution structure of an RNA duplex including a C-U base pair", BIOCHEM, 39(24), 2000, pp. 7074-7080

Abstract

The formation of the C-U base pair in a duplex was observed in solution bymeans of the temperature profile of N-15 chemical shifts, and the precise geometry of the C-U base pair was also determined by NOE-based structure calculation. From the solution structure of the RNA oligomer, r[CCTACUCAGG] .r[CCUGCGUCG], it was found that a single C-U mismatch preferred being stacked in the duplex rather than being flipped-out even in solution. Moreover, it adopts an irregular geometry, where the amino nitrogen (N4) of the cytidine and keto-oxygen (O4) of the uridine are within hydrogen-bonding distance, as seen in crystals. To further prove the presence of a hydrogen bond inthe C-U pair, we employed a point-labeled cytidine at the exocyclic amino nitrogen of the cytidine in the C-U pair. The temperature profile of its N-15 chemical shift showed a sigmoidal transition curve, indicating the presence of a hydrogen bond in the C-U pair in the duplex.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/07/20 alle ore 10:43:15