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Titolo:
PPAR-gamma is selectively upregulated in Caco-2 cells by butyrate.
Autore:
Wachtershauser, A; Loitsch, SM; Stein, J;
Indirizzi:
Univ Frankfurt, Dept Med 2, D-60590 Frankfurt, Germany Univ Frankfurt Frankfurt Germany D-60590 d 2, D-60590 Frankfurt, Germany
Titolo Testata:
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
fascicolo: 2, volume: 272, anno: 2000,
pagine: 380 - 385
SICI:
0006-291X(20000607)272:2<380:PISUIC>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACTIVATED RECEPTOR-GAMMA; ADIPOCYTE DIFFERENTIATION; COLORECTAL-CARCINOMA; MESSENGER-RNA; COLON-CANCER; EXPRESSION; APOPTOSIS; PROMOTES; LIGANDS; GROWTH;
Keywords:
butyrate; Caco-2 cells; differentiation; PPAR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Wachtershauser, A Univ Frankfurt, Dept Med 2, Theodor Stern Kai 7, D-60590Frankfurt, Germany Univ Frankfurt Theodor Stern Kai 7 Frankfurt Germany D-60590
Citazione:
A. Wachtershauser et al., "PPAR-gamma is selectively upregulated in Caco-2 cells by butyrate.", BIOC BIOP R, 272(2), 2000, pp. 380-385

Abstract

The peroxisome proliferator-activated receptor (PPAR)-gamma is a nuclear lipid-activable receptor controlling the expression of genes involved in lipid metabolism and adipocyte differentiation. In order to investigate the possible role of PPAR-gamma in the differentiation of intestinal epithelial cells, we examined its expression in the human colon carcinoma cell line Caco-2, which undergoes rapid cell differentiation in the presence of butyrate. PPARs were quantified on mRNA level by RT competitive multiplex PCR, the corresponding proteins were determined by Western blot. In contrast to PPAR-alpha and PPAR-beta, PPAR-gamma mRNA and protein increased significantly in butyrate-treated Caco-2 cells in a dose- and time-dependent manner. This effect was butyrate-specific, since no change in PPAR-gamma expression could be observed after incubation with propionate or valerate, Activation of PPAR-gamma by ciglitazone further increased butyrate-induced cell differentiation dose-dependently. These data demonstrate a role for PPAR-gamma in theregulation of cell differentiation in Caco-2 cells. (C) 2000 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/09/20 alle ore 09:23:22