Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Identification of high density lipoprotein-binding proteins, including a glycosyl phosphatidylinositol-anchored membrane dipeptidase, in rat lung andtype II pneumocytes
Autore:
Witt, W; Kolleck, I; Rustow, B;
Indirizzi:
Humboldt Univ, Dept Med, D-10117 Berlin, Germany Humboldt Univ Berlin Germany D-10117 , Dept Med, D-10117 Berlin, Germany
Titolo Testata:
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY
fascicolo: 6, volume: 22, anno: 2000,
pagine: 739 - 746
SICI:
1044-1549(200006)22:6<739:IOHDLP>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
SURFACTANT CHOLESTEROL-METABOLISM; SITE-DIRECTED MUTAGENESIS; RENAL DIPEPTIDASE; VITAMIN-E; RECEPTORS; CELLS; CLONING; PORCINE; SERUM; LIVER;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
48
Recensione:
Indirizzi per estratti:
Indirizzo: Rustow, B Humboldt Univ, Univ Hosp Charite, Dept Neonatol, Schumannstr 20-21, D-10117 Berlin, Germany Humboldt Univ Schumannstr 20-21 Berlin GermanyD-10117 Germany
Citazione:
W. Witt et al., "Identification of high density lipoprotein-binding proteins, including a glycosyl phosphatidylinositol-anchored membrane dipeptidase, in rat lung andtype II pneumocytes", AM J RESP C, 22(6), 2000, pp. 739-746

Abstract

Numerous communications have indicated that specific binding proteins for high density lipoprotein (HDL) exist in addition to the well characterized candidate HDL receptor SR-BI, but structural information was presented onlyin a few cases, and most of the work was aimed at the liver and steroidogenic glands. In this study, we purified two HDL-binding proteins by standardprocedures from rat lung tissue. One of these membrane glycoproteins was identified by N-terminal sequencing and with specific antibodies as HB2, a previously described HDL-binding protein, whereas the other one was identified as a glycosyl phosphatidylinositol-anchored membrane dipeptidase (MDP), The apparent dissociation constant of the HDL binding was determined by solid phase assay to be 2.1 mu g/ml (HB2) and 25 mu g/ml (MDP). MDP also exerts affinity to low density lipoprotein (LDL) on ligand blots, and competition between HDL and LDL was observed, but analysis by solid phase assay showed that very high concentrations of LDL are required. The physiologic relevance of this effect is therefore questionable. The level in type II pneumocyte membranes of both binding proteins, MDP and HB2, increased when the plasma lipoprotein concentration was reduced by treatment of rats with 4-aminopyrazolo[3,4-d]-pyrimidine, consistent with a function to facilitate lipid uptake in vivo. The binding proteins were also dramatically upregulated by feeding rats a vitamin E-depleted diet. Vitamin E uptake requires interaction between HDL and type II cells, suggesting a role of HB2 and MDP also in this process.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/12/20 alle ore 21:44:13