Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Ethanol-induced c-Fos expression in catecholamine- and neuropeptide Y-producing neurons in rat brainstem
Autore:
Thiele, TE; Cubero, I; van Dijk, G; Mediavilla, C; Bernstein, IL;
Indirizzi:
Univ Washington, Dept Psychol, Seattle, WA 98195 USA Univ Washington Seattle WA USA 98195 Dept Psychol, Seattle, WA 98195 USA Univ Washington, Inst Alcohol & Drug Abuse, Seattle, WA 98195 USA Univ Washington Seattle WA USA 98195 & Drug Abuse, Seattle, WA 98195 USA Univ Almeria, Dept Pscicol Expt & Psicobiol, Almeria, Spain Univ Almeria Almeria Spain ept Pscicol Expt & Psicobiol, Almeria, Spain Univ Groningen, Dept Anim Physiol, Groningen, Netherlands Univ Groningen Groningen Netherlands im Physiol, Groningen, Netherlands Univ Granada, Dept Psicol Expt & Fisiol Comportamiento, Granada, Spain Univ Granada Granada Spain Expt & Fisiol Comportamiento, Granada, Spain
Titolo Testata:
ALCOHOLISM-CLINICAL AND EXPERIMENTAL RESEARCH
fascicolo: 6, volume: 24, anno: 2000,
pagine: 802 - 809
SICI:
0145-6008(200006)24:6<802:ECEICA>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
LOCUS COERULEUS NEURONS; PRESUMPTIVE TERMINAL PROCESSES; PARAVENTRICULAR NUCLEUS; ALCOHOL-CONSUMPTION; MEDULLA-OBLONGATA; NERVOUS-SYSTEM; DEPENDENT RATS; SOLITARY TRACT; HYPOTHALAMUS; STEM;
Keywords:
alcohol; locus caeruleus; neuropeptide Y; norepinephrine; ventrolateral medulla;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
53
Recensione:
Indirizzi per estratti:
Indirizzo: Thiele, TE Univ Washington, Dept Psychol, Box 351525, Seattle, WA 98195 USA Univ Washington Box 351525 Seattle WA USA 98195 e, WA 98195 USA
Citazione:
T.E. Thiele et al., "Ethanol-induced c-Fos expression in catecholamine- and neuropeptide Y-producing neurons in rat brainstem", ALC CLIN EX, 24(6), 2000, pp. 802-809

Abstract

Background: Previous studies have used c-Fos-like immunoreactivity (cFLI) to examine the neuroanatomical location of cells that are activated in response to ethanol administration. However, the use of cFLI alone fails to reveal the phenotypical identity of cells. Tn the present study we used double-labeling procedures to identify the neurochemical phenotype of neurons that showed ethanol-induced cFLI in the rat brainstem. Methods: Individual groups of rats received intraperitoneal injection of ethanol (1.5 g/kg or 3.5 g/kg) or isotonic saline (23 ml/kg). To assess the specificity of cFLI induced by ethanol, we injected other rats with the drug lithium chloride (LiCl; 76 mg/kg).Two hours after injection, rats were killed and their brains were processed for immunohistochemistry. Results: Both doses of ethanol promoted cFLI in several brainstem regions,including the nucleus of the solitary tract (NTS), the locus coeruleus (LC), and the ventrolateral medulla (VLM). Although LiCl caused significant cFTI in the NTS, this drug promoted only minimal cFLI in the VLM and no significant activation in the LC. We found that a significant proportion of tyrosine hydroxylase (TH)-positive neurons coexpressed ethanol-induced cFLI in the VLM (similar to 75-85%), the NTS (similar to 65-75%), and the LC (similar to 30-65%). Additionally, a significant proportion of neuropeptide Y (NPY)-producing neurons in the VLM coexpressed ethanol-induced cFLI (similar to 60-75%). On the other hand, LiCl promoted activation of TW-positive neurons in the VLM and the NTS but failed to stimulate cFLI in TH-producing neurons in the LC or in NPY-producing neurons of the VLMConclusions: Neurons in the rat brainstem that show ethanol-induced c-Fos expression produce catecholamines and NPY. This research demonstrates the usefulness of double-labeling immunohistochemistry procedures for identifying the neurochemical identity of neurons that are activated after ethanol administration.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/09/20 alle ore 12:04:08