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Titolo:
Cloning and analysis of unique human glutaminase isoforms generated by tissue-specific alternative splicing
Autore:
Elgadi, KM; Meguid, RA; Qian, M; Souba, WW; Abcouwer, SF;
Indirizzi:
Massachusetts Gen Hosp, Surg Oncol Res Labs, Boston, MA 02114 USA Massachusetts Gen Hosp Boston MA USA 02114 Res Labs, Boston, MA 02114 USA Harvard Univ, Sch Med, Dept Surg, Boston, MA 02114 USA Harvard Univ Boston MA USA 02114 Sch Med, Dept Surg, Boston, MA 02114 USA
Titolo Testata:
PHYSIOLOGICAL GENOMICS
fascicolo: 2, volume: 1, anno: 1999,
pagine: 51 - 62
SICI:
1094-8341(19990831)1:2<51:CAAOUH>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
MEMBRANE-BOUND FORMS; MITOCHONDRIAL GLUTAMINASE; RAT-KIDNEY; METABOLIC-ACIDOSIS; RENAL GLUTAMINASE; MESSENGER-RNA; AMINO-ACIDS; EXPRESSION; IDENTITY; DECREASE;
Keywords:
glutamine metabolism; mitochondrial proteins; gene expression; mRNA processing; CAG repeat polymorphisms; brain; kidney; muscle; mammary adenocarcinoma;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: Abcouwer, SF Univ New Mexico, Sch Med, Hlth Sci Ctr, Dept Biochem & Mol Biol, Basic MedSci Bldg 249, Albuquerque, NM 87131 USA Univ New Mexico Basic Med Sci Bldg 249 Albuquerque NM USA 87131
Citazione:
K.M. Elgadi et al., "Cloning and analysis of unique human glutaminase isoforms generated by tissue-specific alternative splicing", PHYSIOL GEN, 1(2), 1999, pp. 51-62

Abstract

Three human glutaminase (hGA) isoforms were identified, two of which represent isoforms previously unidentified in any species. One isoform contains an open reading frame with high homology with the rat kidney-type glutaminase, suggesting that this isoform represents the human kidney-type glutaminase, hKGA. A second isoform, termed hGAC, contains an open reading frame that matches hKGA except for a unique COOH-terminal amino acid sequence. In addition, a third human glutaminase isoform was identified from a computer search and on further analysis was found to represent an additional unique isoform, hGAM. hKGA is expressed predominantly in brain and kidney but not inliver, hGAC is expressed principally in cardiac muscle and pancreas but not in liver or brain, and hGAM is expressed solely in cardiac and skeletal muscle. hGAC is the predominant isoform expressed by a human breast cancer cell line that exhibits a high rate of glutamine utilization and glutaminaseactivity. Genomic Southern analysis as well as isolation and analysis of five glutaminase genomic clones suggested that all three hGA isoforms originate from the same locus and therefore represent mRNA species that are produced by tissue-specific alternative splicing of a single pre-mRNA. Furthermore, an RT-PCR assay was developed that can be used to easily differentiate between hKGA and hGAC mRNA species.

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Documento generato il 30/11/20 alle ore 07:18:55