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Titolo:
Studies of in vivo mutations in rpsL transgene in UVB-irradiated epidermisof XPA-deficient mice
Autore:
Murai, H; Takeuchi, S; Nakatsu, Y; Ichikawa, M; Yoshino, M; Gondo, Y; Katsuki, M; Tanaka, K;
Indirizzi:
Osaka Univ, Inst Mol & Cellular Biol, Div Cellular Genet, Suita, Osaka 5650871, Japan Osaka Univ Suita Osaka Japan 5650871 r Genet, Suita, Osaka 5650871, Japan RIKEN, Genom Sci Ctr, Totsuka Ku, Yokohama, Kanagawa 2440804, Japan RIKENYokohama Kanagawa Japan 2440804 , Yokohama, Kanagawa 2440804, Japan Univ Tokyo, Inst Med Sci, Ctr Med Expt, Div DNA Biol & Embryo Engn, Tokyo,Japan Univ Tokyo Tokyo Japan ed Expt, Div DNA Biol & Embryo Engn, Tokyo,Japan Japan Sci & Technol Corp, CREST, Wako, Saitama, Japan Japan Sci & Technol Corp Wako Saitama Japan CREST, Wako, Saitama, Japan
Titolo Testata:
MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS
fascicolo: 1-2, volume: 450, anno: 2000,
pagine: 181 -
SICI:
1386-1964(20000530)450:1-2<181:SOIVMI>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
REPAIR GENE XPA; CYCLOBUTANE PYRIMIDINE DIMERS; INDUCED SKIN TUMORS; XERODERMA-PIGMENTOSUM; DNA-REPAIR; ULTRAVIOLET-B; IN-VIVO; INDUCED MUTAGENESIS; ESCHERICHIA-COLI; MOUSE MODEL;
Keywords:
rpsL gene; UV; mutation; xeroderma pigmentosum; transgenic mice; knockout mice;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Tanaka, K Osaka Univ, Inst Mol & Cellular Biol, Div Cellular Genet, 1-3 Yamadaoka, Suita, Osaka 5650871, Japan Osaka Univ 1-3 Yamadaoka Suita Osaka Japan 5650871 650871, Japan
Citazione:
H. Murai et al., "Studies of in vivo mutations in rpsL transgene in UVB-irradiated epidermisof XPA-deficient mice", MUT RES-F M, 450(1-2), 2000, pp. 181

Abstract

We have established xeroderma pigmentosum group A (XPA) gene-knockout micewith nucleotide excision repair (NER) deficiency, which rapidly developed skin tumors when exposed to a low dose of chronic UV like XP-A patients, confirming that the NER process plays an important role in preventing UVB-induced skin cancer. To examine the in vivo mutation in the UVB-irradiated epidermis, we established XPA(- / -), (+ / -) and(+ / +) mice carrying the Escherichia coil rpsL transgene with which the mutation frequencies and spectra in the UVB-irradiated epidermal tissue can be examined conveniently. The XPA (- / -) mice showed a higher frequency of UVB-induced mutation in the rpsL transgene with a low dose (150 J/m(2)) of UVB-irradiation than the XPA (+ / -) and (+ / -) mice, while, at a high dose (900 J/m(2)) they showed almost the same frequency of mutation as the XPA (+ / -) and (+ / +) mice, probably because of cell death in the epidermis of the XPA (- / -) mice. However, CC --> TT tandem transition, a hallmark of W-induced mutation, was detected at higher frequency in the XPA (- / -) mice than the XPA (+ / -) and (+ / +) mice at both doses of UVB. This rpsL / XPA mouse system will be useful for further analyzing the role of NER in the mutagenesis and carcinogenesis induced by various carcinogens. (C) 2000 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 13/07/20 alle ore 06:47:46