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Titolo:
Cyanide inhibits respiration yet stimulates aerobic growth of Zymomonas mobilis
Autore:
Kalnenieks, U; Galinina, N; Toma, MM; Poole, RK;
Indirizzi:
Univ Sheffield, Krebs Inst Biomolec Res, Dept Mol Biol & Biotechnol, Sheffield S10 2TN, S Yorkshire, England Univ Sheffield Sheffield S Yorkshire England S10 2TN S Yorkshire, England Latvian State Univ, Inst Microbiol & Biotechnol, LV-1586 Riga, Latvia Latvian State Univ Riga Latvia LV-1586 Biotechnol, LV-1586 Riga, Latvia
Titolo Testata:
MICROBIOLOGY-UK
, volume: 146, anno: 2000,
parte:, 6
pagine: 1259 - 1266
SICI:
1350-0872(200006)146:<1259:CIRYSA>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
ELECTRON-TRANSPORT CHAIN; ESCHERICHIA-COLI; OXIDATIVE-PHOSPHORYLATION; OXYGEN; MEMBRANE; OXIDASE; ACETALDEHYDE; MUTAGENESIS; DEFICIENT; STRAINS;
Keywords:
Zymomonas mobilis; cyanide sensitivity; respiratory protection; cytochromes; acetaldehyde;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Poole, RK Univ Sheffield, Krebs Inst Biomolec Res, Dept Mol Biol & Biotechnol, FirthCourt,WEstern Bank, Sheffield S10 2TN, S Yorkshire, England Univ Sheffield Firth Court,WEstern Bank Sheffield S Yorkshire England S10 2TN
Citazione:
U. Kalnenieks et al., "Cyanide inhibits respiration yet stimulates aerobic growth of Zymomonas mobilis", MICROBIO-UK, 146, 2000, pp. 1259-1266

Abstract

Potassium cyanide at submillimolar concentrations (20-500 mu M) inhibited the high respiration rates of aerobic cultures of Zymomonas mobilis but, remarkably, stimulated culture growth, In batch culture, after an extended lag phase, exponential growth persisted longer, resulting in higher biomass densities. In aerobic chemostat cultures, elevated biomass concentration wasobserved in the presence of cyanide, This growth stimulation effect is attributed to decreased production of the inhibitory metabolite acetaldehyde at lowered respiration rates, when more reducing equivalents are channelled to alcohol dehydrogenase. Growth in the presence of cyanide did not alter the membrane cytochrome content. In non-growing cyanide-preincubated cells, with ethanol as the respiratory substrate, cyanide increased ATP levels; insuch cells, a large part of the cyanide-sensitive respiration was inhibited within a few seconds after ethanol addition, while inhibition of the restof respiration took several minutes, The more cyanide-sensitive respiration was apparently energy-nongenerating, and was absent in membrane preparations, Pelleting of membranes from cell-free extracts produced 'soluble' fractions in which a b-type haem was detectable by reduced minus oxidized difference spectroscopy, The function of the Z. mobilis respiratory chain in cell growth and respiratory protection, and the possible physiological role ofaerobic generation of inhibitory metabolites, are discussed.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/01/20 alle ore 10:00:45