Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Effects of a high-fat-sucrose diet on enzymes in homocysteine metabolism in the rat
Autore:
Fonseca, V; Dicker-Brown, A; Ranganathan, S; Song, W; Barnard, RJ; Fink, L; Kern, PA;
Indirizzi:
Univ Arkansas Med Sci, Little Rock Cent Arkansas Vet Healthcare Syst, DeptMed, Div Endocrinol, Little Rock, AR 72205 USA Univ Arkansas Med Sci Little Rock AR USA 72205 Little Rock, AR 72205 USA Univ Arkansas Med Sci, Little Rock Cent Arkansas Vet Healthcare Syst, DeptPathol, Little Rock, AR 72205 USA Univ Arkansas Med Sci Little Rock AR USA72205 Little Rock, AR 72205 USA Univ Calif Los Angeles, Dept Physiol Sci, Los Angeles, CA USA Univ Calif Los Angeles Los Angeles CA USA ysiol Sci, Los Angeles, CA USA
Titolo Testata:
METABOLISM-CLINICAL AND EXPERIMENTAL
fascicolo: 6, volume: 49, anno: 2000,
pagine: 736 - 741
SICI:
0026-0495(200006)49:6<736:EOAHDO>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
CORONARY-ARTERY DISEASE; DEPENDENT DIABETES-MELLITUS; INSULIN-RESISTANCE; METHYLENETETRAHYDROFOLATE REDUCTASE; PLASMA HOMOCYSTEINE; SKELETAL-MUSCLE; METHIONINE LOAD; RISK FACTOR; HYPERHOMOCYSTEINEMIA; HYPERINSULINEMIA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: Fonseca, V Tulane Univ, Sch Med, Dept Med, Sect Endocrinol SL53, 1430 Tulane Ave, NewOrleans, LA 70112 USA Tulane Univ 1430 Tulane Ave New Orleans LAUSA 70112 70112 USA
Citazione:
V. Fonseca et al., "Effects of a high-fat-sucrose diet on enzymes in homocysteine metabolism in the rat", METABOLISM, 49(6), 2000, pp. 736-741

Abstract

Hyperhomocysteinemia (HH) and hyperinsulinemia are both risk factors for cardiovascular disease. To examine the effects of hyperinsulinemia on homocysteine metabolism, we fed rats a high-fat-sucrose (HFS) diet and then measured the hepatic mRNA and activity of 2 key enzymes involved in this metabolic pathway: 5,10-methylenetetrahydrofolate reductase (MTHFR) and cystathionine-beta-synthase (C beta S). Fischer rats made insulin-resistant by a HFS diet were examined at 6 months and 2 years of age and compared with controlrats fed a low-fat, complex-carbohydrate (LFCC) diet. At the end of 6 months, the HFS rats were heavier than the LFCC rats (214 +/- 3.4 v 188 +/- 1.4g, P < .01). There were no differences in blood glucose between HFS and LFCC rats; however, plasma insulin and homocysteine concentrations were elevated in HFS rats (insulin, 56 +/- 12 v 14.5 +/- 2.9 mu U/mL; homocysteine, 10.77 +/- 0.9 v 6.89 +/- 0.34 mu mol/L, P < .01). Hepatic CPS enzyme activity was significantly lower in HFS compared with LFCC rats (0.45 v 0.64 U/mg,P = .0001), and this decrease was reflected in a decrease of the CPS mRNA concentration, In contrast, hepatic MTHFR enzyme activity and mRNA concentration were significantly elevated in the HFS group compared with controls (HFS and LFCC, 8.62 and 4.8 nmol/h/mg protein, respectively, P = .0001). These changes in plasma homocysteine, CPS, and MTHFR were significantly correlated with the degree of obesity and hyperinsulinemia. Fasting plasma insulin correlated significantly and positively with plasma homocysteine (r = .51, P < .01) and MTHFR activity (r = .48, P < .01) and negatively with CPS activity (r = -.54, P < .001). CPS and MTHFR activities were inversely correlated with each other (r = -.58, P < .001), In conclusion, rats fed a HFS diet are hyperinsulinemic, and the hyperinsulinemia is associated with an elevated homocysteine concentration and changes in 2 key enzymes in homocysteine metabolism. Copyright (C) 2000 by W.B. Saunders Company.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/07/20 alle ore 14:23:17