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Titolo:
DNA in situ hybridization on polytene chromosomes of Simulium sanctipauli at loci relevant to insecticide resistance
Autore:
Boakye, DA; Cornel, AJ; Meredith, SE; Brakefield, PM; Collins, FH;
Indirizzi:
Leiden Univ, Inst Evolutionary & Ecol Sci, Sect Evolutionary Biol, NL-2300RA Leiden, Netherlands Leiden Univ Leiden Netherlands NL-2300 RA NL-2300RA Leiden, Netherlands
Titolo Testata:
MEDICAL AND VETERINARY ENTOMOLOGY
fascicolo: 2, volume: 14, anno: 2000,
pagine: 217 - 222
SICI:
0269-283X(200006)14:2<217:DISHOP>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
ANOPHELES-GAMBIAE COMPLEX; DAMNOSUM THEOBALD DIPTERA; INSITU HYBRIDIZATION; LOCALIZATION; GENE; IDENTIFICATION; MEMBERS; MAP;
Keywords:
Simulium damnosum complex; S. sanctipauli; aspartate amino transferase; chromosome mapping; cytochrome P450; DNA hybridization; polymerase chain reaction; polytene chromosomes; Cote d'Ivoire; Ghana;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: Boakye, DA Univ Ghana, Noguchi Mem Inst Med Res, POB LG581 Legon, Accra, Ghana Univ Ghana POB LG581 Legon Accra Ghana 81 Legon, Accra, Ghana
Citazione:
D.A. Boakye et al., "DNA in situ hybridization on polytene chromosomes of Simulium sanctipauli at loci relevant to insecticide resistance", MED VET ENT, 14(2), 2000, pp. 217-222

Abstract

A DNA technique for in situ hybridization developed by Kumar & Collins (1994) for use on polytene chromosomes of adult Anopheles mosquitoes (Diptera:Culicidae) was modified for use with Simulium larval salivary gland chromosomes (Diptera: Simuliidae). Cloned fragments of several Simulium genes (coding for aspartate amino transferase, cytochrome P450 and DNA polymerase) were successfully mapped physically by assigning specific band locations in Simulim sanctipauli V. & D. This represents the first attempt at locating genes beyond the resolution of linkage to inversions in any blackfly species.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/09/20 alle ore 10:33:10