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Titolo:
Developmental expression of dermatan sulfate proteoglycans in the elastic bovine nuchal ligament
Autore:
Reinboth, BJ; Finnis, ML; Gibson, MA; Sandberg, LB; Cleary, EG;
Indirizzi:
Univ Adelaide, Dept Pathol, Adelaide, SA 5005, Australia Univ Adelaide Adelaide SA Australia 5005 ol, Adelaide, SA 5005, Australia Jerry L Pettis Mem Vet Hosp, Loma Linda, CA 92367 USA Jerry L Pettis Mem Vet Hosp Loma Linda CA USA 92367 a Linda, CA 92367 USA
Titolo Testata:
MATRIX BIOLOGY
fascicolo: 2, volume: 19, anno: 2000,
pagine: 149 - 162
SICI:
0945-053X(200005)19:2<149:DEODSP>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
CHONDROITIN/DERMATAN SULFATE; EXTRACELLULAR-MATRIX; CONNECTIVE TISSUES; SEQUENCE-ANALYSIS; BINDING-PROTEIN; MESSENGER-RNA; LYSYL OXIDASE; IDURONIC ACID; MICROFIBRILS; BIGLYCAN;
Keywords:
elastin; biglycan; hyaluronan; microfibrils; dermatan sulfate; nuchal ligament;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
62
Recensione:
Indirizzi per estratti:
Indirizzo: Cleary, EG Univ Adelaide, Dept Pathol, GPO Box 498, Adelaide, SA 5005, Australia Univ Adelaide GPO Box 498 Adelaide SA Australia 5005 Australia
Citazione:
B.J. Reinboth et al., "Developmental expression of dermatan sulfate proteoglycans in the elastic bovine nuchal ligament", MATRIX BIOL, 19(2), 2000, pp. 149-162

Abstract

The nuchal ligament of bovines is a useful system in which to study elastic fibre formation since it contains up to 83% elastin and undergoes a period of rapid elastinogenesis during the last trimester of fetal development and in the first four post-natal months. To identify proteoglycans (PGs) which may be involved in this process we initially investigated changes in theglycosaminoglycan (GAG) profiles during nuchal ligament development. In contrast to the collagenous Achilles tendon, nuchal ligament exhibited: (a) elevated hyaluronan (HA) levels in the peak period of elastin-associated microfibril (fibrillin) synthesis (130-200 days) which precedes elastinogenesis; and (b) markedly increased synthesis of a glucuronate-rich copolymeric form of dermatan sulfate (DS) in the period corresponding to elastin formation (200-270 days). Analysis of DSPGs isolated from 230-day nuchal ligament showed that this copolymer was predominantly associated with a glycoform ofbiglycan which was specifically elevated at this stage in development. This finding was consistent with Northern blot analysis which showed that steady-state biglycan mRNA levels increased significantly during the elastinogenic period. In contrast, the mRNA levels for decorin, the only other DSPG detected in this tissue, declined rapidly after 140 days of fetal development. In conclusion, the results suggest that HA may play a role in microfibril assembly and that a specific glycoform of biglycan may be associated withthe elastinogenic phase of elastic fibre formation. (C) 2000 Published by Elsevier Science B.V. /International Society of Matrix Biology. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/08/20 alle ore 01:07:01