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Titolo:
Treatment of cells with detergent activates caspases and induces apoptoticcell death
Autore:
Strupp, W; Weidinger, G; Scheller, C; Ehret, R; Ohnimus, H; Girschick, H; Tas, P; Flory, E; Heinkelein, M; Jassoy, C;
Indirizzi:
Univ Wurzburg, Inst Virol & Immunobiol, D-97078 Wurzburg, Germany Univ Wurzburg Wurzburg Germany D-97078 nobiol, D-97078 Wurzburg, Germany Univ Wurzburg, Childrens Hosp, D-97078 Wurzburg, Germany Univ Wurzburg Wurzburg Germany D-97078 s Hosp, D-97078 Wurzburg, Germany Univ Wurzburg, Dept Anaesthesiol, D-97078 Wurzburg, Germany Univ WurzburgWurzburg Germany D-97078 hesiol, D-97078 Wurzburg, Germany Univ Wurzburg, Inst Med Radiat & Cell Res, D-97078 Wurzburg, Germany Univ Wurzburg Wurzburg Germany D-97078 ll Res, D-97078 Wurzburg, Germany
Titolo Testata:
JOURNAL OF MEMBRANE BIOLOGY
fascicolo: 3, volume: 175, anno: 2000,
pagine: 181 - 189
SICI:
0022-2631(20000601)175:3<181:TOCWDA>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
TUMOR NECROSIS FACTOR; TOXIC LYMPHOCYTE-T; DNA FRAGMENTATION; MONOCLONAL-ANTIBODY; ANTICANCER DRUGS; INDUCTION; CLEAVAGE; CALCIUM; TARGET; HYPERTHERMIA;
Keywords:
detergent; Triton X-100; Nonidet P-40; n-octylglucoside; sodium deoxycholate; apoptosis; caspase; Jurkat T lymphoblast;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
43
Recensione:
Indirizzi per estratti:
Indirizzo: Jassoy, C Univ Wurzburg, Inst Virol & Immunobiol, Versbacher Str 7, D-97078 Wurzburg, Germany Univ Wurzburg Versbacher Str 7 Wurzburg Germany D-97078 Germany
Citazione:
W. Strupp et al., "Treatment of cells with detergent activates caspases and induces apoptoticcell death", J MEMBR BIO, 175(3), 2000, pp. 181-189

Abstract

Due to their amphiphilic properties, detergents readily disrupt cellular membranes and cause rapid cytolysis. In this study we demonstrate that treatment of cells with sublytic concentrations of detergents such as Triton X-100, Nonidet- P-30, n-octylglucoside and the bile salt sodium deoxycholate induce typical signs of apoptosis including DNA fragmentation and cleavage of poly(ADP-ribose) polymerase molecules. The detergent concentration required for apoptosis was below the critical micellar concentration. Induction of apoptosis was not restricted to human cells but similarly occurred in a variety of other vertebrate cell lines. Unstimulated peripheral blood mononuclear cells were susceptible to apoptosis induction by detergent suggestingthat apoptosis in this circumstance is not mediated by CD95. Cell death was not due to influx of calcium from the medium. Apoptosis was blocked and cytolysis prevented by treatment with peptide inhibitors of caspases. These findings suggest a process of apoptosis that is initiated upon nonspecific alterations at the cell membrane level. Physiologic correlates of this process still have to be defined.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/09/20 alle ore 20:49:10