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Titolo:
An immortalized rat liver stellate cell line (HSC-T6): a new cell model for the study of retinoid metabolism in vitro
Autore:
Vogel, S; Piantedosi, R; Frank, J; Lalazar, A; Rockey, DC; Friedman, SL; Blaner, WS;
Indirizzi:
Columbia Univ, Coll Phys & Surg, Dept Med, New York, NY 10032 USA ColumbiaUniv New York NY USA 10032 urg, Dept Med, New York, NY 10032 USA Columbia Univ, Coll Phys & Surg, Dept Dermatol, New York, NY 10032 USA Columbia Univ New York NY USA 10032 Dept Dermatol, New York, NY 10032 USA Columbia Univ, Coll Phys & Surg, Inst Human Nutr, New York, NY 10032 USA Columbia Univ New York NY USA 10032 st Human Nutr, New York, NY 10032 USA CUNY Mt Sinai Sch Med, Div Liver Dis, New York, NY 10029 USA CUNY Mt SinaiSch Med New York NY USA 10029 r Dis, New York, NY 10029 USA Duke Univ, Med Ctr, Duke Liver Ctr, Durham, NC 27710 USA Duke Univ DurhamNC USA 27710 d Ctr, Duke Liver Ctr, Durham, NC 27710 USA
Titolo Testata:
JOURNAL OF LIPID RESEARCH
fascicolo: 6, volume: 41, anno: 2000,
pagine: 882 - 893
SICI:
0022-2275(200006)41:6<882:AIRLSC>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
FAT-STORING CELLS; ACID RESPONSE ELEMENT; BINDING PROTEIN; VITAMIN-A; ACYLTRANSFERASE ACTIVITY; LIPID-COMPOSITION; ESTER HYDROLASES; ESTERIFICATION; EXPRESSION; TRANSTHYRETIN;
Keywords:
hepatic cells; Ito cells; fat-storing cells; vitamin A; oleic acid; cellular retinol-binding protein, type I (CRBP-1); retinol-binding protein (RBP); transthyretin (TTR); Hepa-1 hepatocytes; retinoid nuclear receptor;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
54
Recensione:
Indirizzi per estratti:
Indirizzo: Blaner, WS Columbia Univ, Coll Phys & Surg, Dept Med, New York, NY 10032 USA Columbia Univ New York NY USA 10032 ed, New York, NY 10032 USA
Citazione:
S. Vogel et al., "An immortalized rat liver stellate cell line (HSC-T6): a new cell model for the study of retinoid metabolism in vitro", J LIPID RES, 41(6), 2000, pp. 882-893

Abstract

Hepatocytes and hepatic stellate cells play important roles in retinoid storage and metabolism. Hepatocytes process postprandial retinyl esters and are responsible for secretion of retinol bound to retinol-binding protein (RBP) to maintain plasma retinol levels. Stellate cells are the body's major cellular storage sites for retinoid, We have characterized and utilized an immortalized rat stellate cell line, HSC-T6 cells, to facilitate study of the cellular aspects of hepatic retinoid processing. For comparison, we alsocarried out parallel studies in Hepa-1 hepatocytes, Like activated primarystellate cells, HSC-T6 express myogenic and neural crest cytoskeletal filaments. HSC-T6 cells take up and esterify retinol in a time- and concentration-dependent manner. Supplementation of HSC-T6 culture medium with free fatty acids (up to 300 mu M) does not affect retinol uptake but does enhance retinol esterification up to 10-fold, RT-PCR analysis indicates that HSC-T6 cells express all 6 retinoid nuclear receptors (RAR alpha, -beta, -gamma, and RXR alpha, -beta, -gamma) and like primary stellate cells, HSC-T6 stellate cells express cellular retinol-binding protein, type I (CRBP) but fail to express either retinol-binding protein (RBP) or transthyretin (TTR). Addition of retinol (10(-8)-10(-5) M) or all-trans-retinoic acid (10(-10)-10(-6) M) rapidly up-regulates CRBP expression, Using RAR-specific agonists and antagonists and an RAR-specific agonist, we show that members of the RAR-receptor family modulate HSC-T6 CRBP expression. jlr Thus, HSC-T6 cells display the same retinoid-related phenotype as primary stellate cells in cultureand will be a useful tool for study of hepatic retinoid storage and metabolism.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/03/20 alle ore 19:18:07