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Titolo:
CD40-ligated dendritic cells effectively expand melanoma-specific CD8(+) CTLs and CD4(+) IFN-gamma-producing T cells from tumor-infiltrating lymphocytes
Autore:
Terheyden, P; Straten, PT; Brocker, EB; Kampgen, E; Becker, JC;
Indirizzi:
Univ Wurzburg, Sch Med, Dept Dermatol, D-97080 Wurzburg, Germany Univ Wurzburg Wurzburg Germany D-97080 rmatol, D-97080 Wurzburg, Germany Danish Canc Soc, Dept Tumor Cell Biol, Copenhagen, Denmark Danish Canc Soc Copenhagen Denmark Tumor Cell Biol, Copenhagen, Denmark
Titolo Testata:
JOURNAL OF IMMUNOLOGY
fascicolo: 12, volume: 164, anno: 2000,
pagine: 6633 - 6639
SICI:
0022-1767(20000615)164:12<6633:CDCEEM>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
CYTOKINE MESSENGER-RNA; LEVEL IL-12 PRODUCTION; CD40 LIGAND; IMMUNE-RESPONSE; HELPER CELL; ANTIGEN; ACTIVATION; PEPTIDE; MICE; INTERLEUKIN-12;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Becker, JC Univ Wurzburg, Sch Med, Dept Dermatol, Josef Schneider Str 2, D-97080 Wurzburg, Germany Univ Wurzburg Josef Schneider Str 2 Wurzburg Germany D-97080 y
Citazione:
P. Terheyden et al., "CD40-ligated dendritic cells effectively expand melanoma-specific CD8(+) CTLs and CD4(+) IFN-gamma-producing T cells from tumor-infiltrating lymphocytes", J IMMUNOL, 164(12), 2000, pp. 6633-6639

Abstract

Professional APC, notably dendritic cells (DC), are necessary for stimulation and expansion of naive T cells. By means of murine models, the interaction between CD40 on DC and its ligand CD154 has been recognized as an important element for conditioning of DC to prime and expand CTL, We translated these findings into the human system, scrutinizing the ability of DC to initiate clonal expansion of single T cells. DC generated under completely autologous conditions from peripheral blood monocytes were cocultured at a rate of 0.3 cell/well with melanoma-infiltrating T cells; this procedure guaranteed that either a CD4(+) or a CD8(+) cell interacted with the DC, thus avoiding the contact of more than one T cell to the DC. In the absence of further stimulation, this cloning protocol yielded almost exclusively CD4(+) Tcell clones that predominantly exhibited a Th2 phenotype, However, cross-linking of CD40 on DC resulted in the induction of IFN-gamma-producing Th1 CD4(+) T cell clones, In addition, CD40-activated DC were capable of expanding CD8(+) CTL clones. The ratio of CD4 to CD8 T cell clones corresponded tothe ratio present in the initial tumor-infiltrating lymphocyte preparation. The CTL clones efficiently lysed autologous tumor cells whereas autologous fibroblasts or MHC-mismatched melanoma cells were not killed. Our findings support the critical role of CD40/CD154 interactions for the induction ofcellular immune responses.

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Documento generato il 04/12/20 alle ore 03:54:56