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Titolo:
Detection of vascular endothelial growth factor and its receptor expression in human hepatocellular carcinoma biopsy specimens
Autore:
Shimamura, T; Saito, S; Morita, K; Kitamura, T; Morimoto, M; Kiba, T; Numata, K; Tanaka, K; Sekihara, H;
Indirizzi:
Yokohama City Univ, Sch Med, Dept Internal Med 3, Yokohama, Kanagawa 2360004, Japan Yokohama City Univ Yokohama Kanagawa Japan 2360004 anagawa 2360004, Japan
Titolo Testata:
JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY
fascicolo: 6, volume: 15, anno: 2000,
pagine: 640 - 646
SICI:
0815-9319(200006)15:6<640:DOVEGF>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
TUMOR ANGIOGENESIS; FACTOR GENE; CANCER; LIVER;
Keywords:
flt-1; hepatocellular carcinoma; KDR; vascular endothelial growth factor; VEGF;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Saito, S 3-9 Fukuura,Kanazawa Ku, Yokohama, Kanagawa 2360004, Japan 3-9 Fukuura,Kanazawa Ku Yokohama Kanagawa Japan 2360004 4, Japan
Citazione:
T. Shimamura et al., "Detection of vascular endothelial growth factor and its receptor expression in human hepatocellular carcinoma biopsy specimens", J GASTR HEP, 15(6), 2000, pp. 640-646

Abstract

Background: Vascular endothelial growth factor (VEGF) exerts its actions on the microvasculature, by interacting with specific endothelial cell receptors, and thus, contributes to angiogenesis and growth in many tumours. Methods: Using nested reverse-transcription-polymerase chain reaction, we examined the biopsy specimens of 14 patients with human hepatocellular carcinoma (HCC) and cirrhosis, for the expression of hepatic VEGF, and the VEGFreceptors KDR and flt-1. To avoid the influence of hypoxia or ischaemia induced by surgical manipulation, we used biopsy specimens of the liver instead of resected specimens. Results: Vascular endothelial growth factor mRNA expression was detected in the tumour portion of the specimens in 13 of 14 patients (93%), and in the corresponding non-tumour portion of the specimens in eight patients (57%;P = 0.08). No differences were found between the tumour portion and the corresponding non-tumour portion in relative concentrations of VEGF mRNA. However, mRNA expression of the VEGF receptors, KDR and flt-1, was detected in14 (100%) and 11 (79%) of the tumour portions, respectively, and in four (29%) and five (36%) of the corresponding non-tumour portions, respectively (chi(2) test: KDR, P < 0.01; flt-1, P = 0.08). The relative concentration of KDR mRNA in the tumour portions was significantly higher than in the non-tumour portions (Mann-Whitney U-test: P < 0.001) but no differences were detected for flt-1. Conclusions: KDR mRNA is significantly overexpressed in HCC lesions and could be associated with the angiogenesis and tumour growth induced by VEGF. (C) 2000 Blackwell Science Asia Pty Ltd.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 00:53:25