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Titolo:
Differentially painting human chromosome arms with combined binary ratio-labeling fluorescence in situ hybridization
Autore:
Wiegant, J; Bezrookove, V; Rosenberg, C; Tanke, HJ; Raap, AK; Zhang, HG; Bittner, M; Trent, JM; Meltzer, P;
Indirizzi:
Leiden Univ, Ctr Med, Dept Mol Cell Biol, Lab Cytochem & Cytometry, Leiden, Netherlands Leiden Univ Leiden Netherlands ytochem & Cytometry, Leiden, Netherlands Natl Human Genome Res Inst, Canc Genet Lab, NIH, Bethesda, MD 20892 USA Natl Human Genome Res Inst Bethesda MD USA 20892 , Bethesda, MD 20892 USA
Titolo Testata:
GENOME RESEARCH
fascicolo: 6, volume: 10, anno: 2000,
pagine: 861 - 865
SICI:
1088-9051(200006)10:6<861:DPHCAW>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
INSITU HYBRIDIZATION; MULTIPLE; PROBES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
10
Recensione:
Indirizzi per estratti:
Indirizzo: Raap, AK Leiden Univ, Ctr Med, Dept Mol Cell Biol, Lab Cytochem & Cytometry, Leiden, Netherlands Leiden Univ Leiden Netherlands Cytometry, Leiden, Netherlands
Citazione:
J. Wiegant et al., "Differentially painting human chromosome arms with combined binary ratio-labeling fluorescence in situ hybridization", GENOME RES, 10(6), 2000, pp. 861-865

Abstract

Recently we developed a novel strategy for differentially painting all 24 human chromosomes. It is termed COBRA-FISH, short for combined binary ratiolabeling-fluorescence in situ hybridization. COBRA-FISH is distinct from the pure combinatorial approach in that only 4 instead of 5 fluorophores areneeded to achieve color discrimination of 24 targets. Furthermore, multiplicity can be increased to 48 by introduction of a fifth Fluorophore. Here we show that color identification by COBRA-FISH of all of the p and q arms of human chromosomes is feasible, and we apply the technique for detecting and elucidating intra- and interchromosomal rearrangements. Compared with 24-color whole chromosome painting FISH, PQ-COBRA-FISH considerably enhances the ability to determine the composition of rearranged chromosomes as demonstrated by the identification of pericentric inversions and isochromosomes as well as the elucidation of the arm identity of chromosomal material involved in complex translocations that occur in solid tumors.

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Documento generato il 30/11/20 alle ore 11:51:38