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Titolo:
Plasma-sulfated C-21-steroids increase during the periovulatory period in female common wolffish and are influenced by temperature during vitellogenesis
Autore:
Tveiten, H; Scott, AP; Johnsen, HK;
Indirizzi:
Univ Tromso, Norwegian Coll Fishery Sci, N-9037 Tromso, Norway Univ Tromso Tromso Norway N-9037 Coll Fishery Sci, N-9037 Tromso, Norway Ctr Environm Fisheries & Aquaculture Sci, Lowestoft NR33 OHT, Suffolk, England Ctr Environm Fisheries & Aquaculture Sci Lowestoft Suffolk England NR33 OHT
Titolo Testata:
GENERAL AND COMPARATIVE ENDOCRINOLOGY
fascicolo: 3, volume: 117, anno: 2000,
pagine: 464 - 473
SICI:
0016-6480(200003)117:3<464:PCIDTP>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
PLAICE PLEURONECTES-PLATESSA; FINAL OOCYTE MATURATION; DETECTS C-21 STEROIDS; ANARHICHAS-LUPUS L; SPAWNING BEHAVIOR; GONADAL-STEROIDS; IN-VITRO; STEROIDOGENESIS; OVULATION; 17-ALPHA,20-BETA-DIHYDROXY-4-PREGNEN-3-ONE;
Keywords:
common wolffish; reproduction; periovulatory period; 17,20 beta-dihydroxy-4-pregnen-3-one; 5 beta-pregnan-3 alpha,17,20 beta-triol sulfate; temperature effects;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: Tveiten, H Univ Tromso, Norwegian Coll Fishery Sci, N-9037 Tromso, Norway Univ Tromso Tromso Norway N-9037 y Sci, N-9037 Tromso, Norway
Citazione:
H. Tveiten et al., "Plasma-sulfated C-21-steroids increase during the periovulatory period in female common wolffish and are influenced by temperature during vitellogenesis", GEN C ENDOC, 117(3), 2000, pp. 464-473

Abstract

Plasma concentrations of steroids during the periovulatory period were measured in female common wolffish reared at three different temperatures. Steroids were quantified by radioimmunoassay (RIA). Two "broad-spectrum specificity" RIAs-one which detects C-21-steroids with a 17,20 beta-dihydroxyl configuration (17,20 beta-steroids) and the other which detects C-21-steroidswith a 5 beta-reduced, 3 alpha-hydroxyl configuration (5 beta,3 alpha-steroids)-picked up very large amounts of cross-reacting material (1.7 mu g ml(-1) in one fish) in the sulfate fraction of plasma from ovulating females. Reverse-phase highperformance liquid chromatography and thin-layer chromatography revealed two major steroids: 5 beta-pregnane-3 alpha,17,20 beta-triol (80%) and 5 beta-pregnane-3 beta,17,20 beta-triol (20%). The sulfated forms of these steroids were elevated 4 to 6 days before and during ovulation,compared with those of females in vitellogenic and postspawning condition,in which concentrations were below 2.0 ng ml(-1). In the three groups of fish held at 4, 8, and 12 degrees C during vitellogenesis, but returned to 4degrees C just prior to the spawning season, the mean concentrations of sulfated 17,20 beta-steroids in ovulating females were 530, 635, and 325 ng ml(-1), respectively. The corresponding concentrations of free 17,20 beta-dihydroxy-4-pregnen-3-one (17,20 beta-P; the maturation-inducing steroid in many teleosts) were 0.88, 0.86, and 0.57 ng ml(-1), respectively. Only minute amounts of 17,20 beta,21-P and its sulfated derivatives were detected. Significantly lower steroid concentrations in the 12 degrees C group indicatethat steroid synthesis and/or metabolism during the periovulatory period are influenced by the temperature experienced during vitellogenesis. In malefish, plasma concentrations of both sulfated 17,20 beta-steroids and free 17,20 beta-P were low (<2.0 ng ml(-1)) at all times. (C) 2000 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/09/20 alle ore 16:06:03