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Titolo:
Passive immunization with antisperm monoclonal antibody ZAP-7 increases preimplantation embryo mortality in the mouse
Autore:
Fuentes, P; Martinez, P;
Indirizzi:
Univ Autonoma Barcelona, Inst Biol Fundamental, Immunol Unit, E-08193 Barcelona, Spain Univ Autonoma Barcelona Barcelona Spain E-08193 E-08193 Barcelona, Spain
Titolo Testata:
FERTILITY AND STERILITY
fascicolo: 6, volume: 73, anno: 2000,
pagine: 1126 - 1131
SICI:
0015-0282(200006)73:6<1126:PIWAMA>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN SPERM; INHIBITION; FERTILITY;
Keywords:
FA-2 antigen; antisperm monoclonal antibody; VIC-1; ZAP-7; passive immunization; fertility inhibition; preimplantation embryo mortality;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
12
Recensione:
Indirizzi per estratti:
Indirizzo: Martinez, P Univ Autonoma Barcelona, Inst Biol Fundamental, Immunol Unit, E-08193 Barcelona, Spain Univ Autonoma Barcelona Barcelona Spain E-08193 elona, Spain
Citazione:
P. Fuentes e P. Martinez, "Passive immunization with antisperm monoclonal antibody ZAP-7 increases preimplantation embryo mortality in the mouse", FERT STERIL, 73(6), 2000, pp. 1126-1131

Abstract

Objective: To evaluate the effect of VIC-1 and ZAP-7 antihuman sperm monoclonal antibodies on in vivo fertility in the mouse. Design: A randomized blinded study using a mouse model. Setting: University-based laboratory. Animals: B6CBAF1 mice (n = 6 per experimental group). Intervention(s): Antisperm antibodies were administered intravaginally to female mice before mating. Control mice received no treatment, saline, or nonspecific antibodies. Number and viability of preimplantation embryos were determined by microscopic observation. Mouse sperm, oocytes, and normal preimplantation embryos were used in indirect immunofluorescence assays with antisperm antibodies. The effect of antibody treatment on sperm motility and vitality was evaluated. Main Outcome Measure(s): Antigen expression, sperm motility and vitality, number and viability of embryos. Result(s): ZAP-7 antibody recognizes a sperm antigen expressed in zygotes and early preimplantation embryos. Passive immunization with ZAP-7 increases embryo mortality significantly (more than 40% above controls). Passive immunization with VIC-1 has no deleterious effect. Conclusion(s): ZAP-7 monoclonal antibody disrupts fertilization and embryogenesis in the mouse. (C) 2000 by American Society for Reproductive Medicine.

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Documento generato il 05/04/20 alle ore 07:01:25