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Titolo:
Characterization of expanded T cell clones in healthy macaques: ontogeny, distribution and stability
Autore:
Nam, KH; Illes, Z; Terao, K; Yoshikawa, Y; Yamamura, T;
Indirizzi:
Natl Inst Infect Dis, Tsukuba Primate Ctr, Tsukuba, Ibaraki 3050843, JapanNatl Inst Infect Dis Tsukuba Ibaraki Japan 3050843 Ibaraki 3050843, Japan Univ Tokyo, Fac Agr, Dept Biomed Sci, Bunkyo Ku, Tokyo 1138657, Japan UnivTokyo Tokyo Japan 1138657 omed Sci, Bunkyo Ku, Tokyo 1138657, Japan NCNP, Natl Inst Neurosci, Dept Demyelinating Dis & Aging, Tokyo 1870031, Japan NCNP Tokyo Japan 1870031 Demyelinating Dis & Aging, Tokyo 1870031, Japan
Titolo Testata:
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
fascicolo: 6-7, volume: 24, anno: 2000,
pagine: 703 - 715
SICI:
0145-305X(200009/10)24:6-7<703:COETCC>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
RECEPTOR-BETA-CHAIN; V-SEGMENT FREQUENCIES; PERIPHERAL-BLOOD; CYNOMOLGUS MONKEYS; MULTIPLE-SCLEROSIS; RHEUMATOID-ARTHRITIS; CLONALITY ANALYSIS; ANTIGEN RECEPTORS; RHESUS-MONKEYS; LYMPHOCYTES-T;
Keywords:
cymolgus monkey; peripheral lymphocyte; T cell; clonal expansion; TCR beta chain; aging; RT-PCRI; SSCP;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
48
Recensione:
Indirizzi per estratti:
Indirizzo: Terao, K Natl Inst Infect Dis, Tsukuba Primate Ctr, 1 Hachimandai, Tsukuba, Ibaraki3050843, Japan Natl Inst Infect Dis 1 Hachimandai Tsukuba Ibaraki Japan 3050843
Citazione:
K.H. Nam et al., "Characterization of expanded T cell clones in healthy macaques: ontogeny, distribution and stability", DEV COMP IM, 24(6-7), 2000, pp. 703-715

Abstract

Peripheral expanded T cell clones have been discussed mainly in relation to certain diseases or immune function in humans and mice. There is little information on their ontogeny, stability and distribution among T cell subsets as well as major lymphoid organs, We applied reverse transcription-polymerase chain reaction (RT-PCR) with family specific primers for monkey T cell receptor beta chain V regions and single-strand conformation polymorphism(SSCP) analysis to analyze the expanded T cell clones in cynomolgus monkeys (Macaca fascicularis). A number of expanded T cell clones were detected in the peripheral blood of young and adult monkeys, but few expanded T cell clones were detected in the blood of a fetus and a 2-day-old neonate. The clones in adults were maintained over 3 months. These expanded T cell cloneswere distributed only in peripheral blood and spleen, but few were found in lymph nodes (axillary, inguinal and intestinal). The number of expanded Tcell clones was much greater in CD8 single-positive (CD8sp) T cells than in CD4sp T cells, showing that most of these clones originated in the CD8sp T cell population. Almost all the expanded CD8sp T cell clones belonged to the CD28(-), CD29(hi) and Fas(+) subset. The usage of V beta genes was not skewed in the 24 V beta. Furthermore, higher mRNA signals for effector molecules perforin and IFN-gamma were detected in CD8sp T cell subsets with phenotypes of CD28-, CD29(hi) and Fas(+), suggesting that the expanded T cellsmight have developed in relation to T cell activation in the periphery of cynomolgus monkeys. (C) 2000 Elsevier Science Ltd. All rights reserved.

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Documento generato il 14/07/20 alle ore 03:31:38