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Titolo:
Antibacterial properties and partial cDNA sequences of cecropin-like antibacterial peptides from the common cutworm, Spodoptera litura
Autore:
Choi, CS; Lee, IH; Kim, E; Kim, SI; Kim, HR;
Indirizzi:
Korea Univ, Dept Biol, Seoul 136701, South Korea Korea Univ Seoul South Korea 136701 Dept Biol, Seoul 136701, South Korea Hoseo Univ, Dept Life Sci, Choongnam 337850, South Korea Hoseo Univ Choongnam South Korea 337850 i, Choongnam 337850, South Korea Korea Basic Sci Inst, Biomol Res Team, Taejon 305333, South Korea Korea Basic Sci Inst Taejon South Korea 305333 aejon 305333, South Korea
Titolo Testata:
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-PHARMACOLOGY TOXICOLOGY & ENDOCRINOLOGY
fascicolo: 3, volume: 125, anno: 2000,
pagine: 287 - 297
SICI:
1367-8280(200003)125:3<287:APAPCS>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
MOLECULAR-CLONING; SARCOPHAGA-PEREGRINA; HYALOPHORA-CECROPIA; INNATE IMMUNITY; INSECT IMMUNITY; BOMBYX-MORI; PROTEINS; MODEL; PURIFICATION; ANTIBIOTICS;
Keywords:
haemolymph protein; fat body; antibacterial peptide; cecropin; RT-PCR; cDNA; northern blot analysis; Spodoptera litura;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: Kim, HR Korea Univ, Dept Biol, Anam Dong 5-1, Seoul 136701, South Korea Korea Univ Anam Dong 5-1 Seoul South Korea 136701 01, South Korea
Citazione:
C.S. Choi et al., "Antibacterial properties and partial cDNA sequences of cecropin-like antibacterial peptides from the common cutworm, Spodoptera litura", COMP BIOC C, 125(3), 2000, pp. 287-297

Abstract

The antibacterial properties and cDNA sequences of two types of antibacterial peptides from the haemolymph of immunized common cutworm, Spodoptera litura larvae, were determined. Since the primary structures of peptides deduced from cDNA sequences showed significant homologies to cecropins A and B:they were named as Spodoptera cecropins A and B. Spodoptera cecropins werebroadly effective against Gram-positive and negative bacteria. They also retained antibacterial activities in all conditions tested (at pH 5.6-8.0 and in the presence of 50-150 mM NaCl) that was adapted to confirm the antibacterial properties of Spodoptera cecropins. These results indicate that thechange of pH and the increase of salt concentration in the media do not influence the activities of Spodoptera cecropins. For the reverse transcription (RT)-polymerase chain reaction (PCR) to obtain the complete primary sequencer the primer designed according to the conserved region of the cecropinleader sequences was used, which was determined by the comparison of the cDNA sequences of known cecropins. The results from RT-PCR presented that the partial cDNAs of Spodoptera cecropins A and B encode 57 and 58 amino acids, including the sequences of mature peptides, respectively. In addition, Northern blot analysis with P-32-labeled PCR product coding for Spodoptera cecropin A revealed that Spodoptera cecropin genes are expressed in immunized fat body, but not in normal fat body, (C) 2000 Elsevier Science Inc. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 15:03:59