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Titolo:
alpha-Amylase from developing embryos of the camel tick Hyalomma dromedarii
Autore:
Mohamed, SA;
Indirizzi:
Natl Res Ctr, Dept Mol Biol, Cairo, Egypt Natl Res Ctr Cairo EgyptNatl Res Ctr, Dept Mol Biol, Cairo, Egypt
Titolo Testata:
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
fascicolo: 1, volume: 126, anno: 2000,
pagine: 99 - 108
SICI:
0305-0491(200005)126:1<99:AFDEOT>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
PURIFICATION; PROTEIN; LARVAE;
Keywords:
camel; tick; Hyalomma dromedarii; embryogenesis; alpha-amylases; purification; characterization;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Mohamed, SA Natl Res Ctr, Dept Mol Biol, Tahrir St, Cairo, Egypt Natl Res Ctr Tahrir St Cairo Egypt , Tahrir St, Cairo, Egypt
Citazione:
S.A. Mohamed, "alpha-Amylase from developing embryos of the camel tick Hyalomma dromedarii", COMP BIOC B, 126(1), 2000, pp. 99-108

Abstract

alpha-Amylase activity in the camel tick Hyalomma dromedarii was followed throughout embryogenesis. During purification of alpha-amylase III to homogeneity, ion exchange chromatography lead to four separate forms (termed I, II, III and IV). alpha-Amylase III with the highest specific activity was pure after chromatography on Sephacryl S-300. The molecular mass of alpha-amylase III was 106 kDa for the native enzyme, composed of two subunits of 43and 66 kDa, respectively. alpha-Amylase had a value of 10 mg starch/ml. Varying alpha-amylase activity was detected when supplied with various substrates. alpha-Amylase III had a temperature optimum at 40 degrees C with heatstability up to 50 degrees C, and a pH optimum of 7.0. The enzyme activitywas activated by CaCl2, MgCl2 and NaNO3, but not activated by NaCl, p-CMB,N-ethylmaleimide and iodoacetamide. EDTA and beta-mercaptoethanol stronglyinhibited activity. (C) 2000 Elsevier Science Inc. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 05:37:30