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Titolo:
ADP-ribosyl cyclase in rat vascular smooth muscle cells - Properties and regulation
Autore:
de Toledo, FGS; Cheng, JF; Liang, MY; Chini, EN; Dousa, TP;
Indirizzi:
Mayo Clin & Mayo Fdn, Mayo Med Sch, Dept Med, Rochester, MN 55905 USA MayoClin & Mayo Fdn Rochester MN USA 55905 Med, Rochester, MN 55905 USA Mayo Clin & Mayo Fdn, Mayo Med Sch, Dept Physiol & Biophys, Div Nephrol & Internal Med, Rochester, MN 55905 USA Mayo Clin & Mayo Fdn Rochester MN USA 55905 Med, Rochester, MN 55905 USA Mayo Clin & Mayo Fdn, Mayo Med Sch, Dept Anesthesiol, Rochester, MN 55905 USA Mayo Clin & Mayo Fdn Rochester MN USA 55905 siol, Rochester, MN 55905 USA
Titolo Testata:
CIRCULATION RESEARCH
fascicolo: 11, volume: 86, anno: 2000,
pagine: 1153 - 1159
SICI:
0009-7330(20000609)86:11<1153:ACIRVS>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
SURFACE ANTIGEN CD38; RETINOIC ACID; NAD(+) GLYCOHYDROLASE; MESANGIAL CELLS; INHIBITION; MITOGENESIS; METABOLISM; RESPONSES; MODULATOR; MOLECULE;
Keywords:
vascular smooth muscle cells; calciferols; antibodies; ADP-ribose; retinoids;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: Chini, EN Mayo Clin & Mayo Fdn, Mayo Med Sch, Dept Med, 200 1st St SW,Guggenheim 9, Rochester, MN 55905 USA Mayo Clin & Mayo Fdn 200 1st St SW,Guggenheim 9 Rochester MN USA 55905
Citazione:
F.G.S. de Toledo et al., "ADP-ribosyl cyclase in rat vascular smooth muscle cells - Properties and regulation", CIRCUL RES, 86(11), 2000, pp. 1153-1159

Abstract

We investigated whether ADP-ribosyl cyclase (ADPR-cyclase) in rat vascularsmooth muscle cells (VSMCs) has enzymatic properties that differ from the well-characterized CD38-antigen ADPR-cyclase, expressed in HL-60 cells. ADPR-cyclase from VSMCs, but not CD38 ADPR-cyclase from HL-60 cells, was inhibited by gangliosides (10 mu mol/L) GT(IB), GD(1), and GM(3). Preincubation of membranes from CD38 HL-60 cells, but not from VSMCs, with anti-CD38 antibodies increased ADPR-cyclase activity; CD38 antigen was detected both in VSMCs and in HL-60 cells. ADPR-cyclase in VSMC membranes was more sensitive than CD38 HL-60 ADPR-cyclase to inactivation by N-endoglycosidase F and to thermal inactivation at 45 degrees C. The specific activity of ADPR-cyclasein membranes from VSMCs was >20-fold higher than in membranes from CD38 HL-60 cells. Most importantly, VSMC ADPR-cyclase was inhibited by Zn2+ and Cu2+ ions; the inhibition by Zn2+ was dose dependent, noncompetitive, and reversible by EDTA. In contrast, Zn2+ stimulated the activity of CD38 HL-60 ADPR-cyclase and other known types of ADPR-cyclases. Retinoids act either viathe nuclear receptor retinoic acid receptor or retinoid X receptor, including all-trans retinoic acid (atRA), and panagonist 9-cis-retinoic acid-upregulated VSMC ADPR-cyclase; the stimulatory effect of atRA was blocked by actinomycin D and cycloheximide. 1,25(OH)(2)-Vitamin D-3 (calciferol) stimulated VSMC ADPR-cyclase dose dependently at subnanomolar concentrations (ED(50)congruent to 56 pmol/L). Oral administration of atRA to rats resulted in an increase of ADPR-cyclase activity in aorta (congruent to+60%) and, to a lesser degree, in myocardium of left ventricle (+18%), but atRA had no effect on ADPR-cyclases in lungs, spleen, intestinal smooth muscle, skeletal muscle, liver, or testis. Administration of 3,5,3'-triiodothyronine (T-3) to rats resulted in an increase of ADPR-cyclase activity in aorta (congruent to+89%), but not in liver or brain. We conclude the following: (1) ADPR-cyclase in VSMCs has enzymatic properties distinct from "classic" CD38 ADPR-cyclase, especially sensitivity to inhibition by Zn2+ and Cu2+;(2) ADPR-cyclase in VSMCs is upregulated by various retinoids, calcitriol, and T-3 in vitro; and (3) administration of atRA and T-3 increases ADPR-cyclase in aorta in vivo. We suggest that the cADPR signaling system plays an important role in the regulation of VSMC functions in response to steroid superfamily hormones.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/11/20 alle ore 08:20:59