Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Application of mathematical modeling to analysis of nerves growth factor interaction with platelets
Autore:
Gurevich, KG; Chekalina, ND; Zakharenko, OM;
Indirizzi:
Moscow MV Lomonosov State Univ, Moscow 119899, Russia Moscow MV Lomonosov State Univ Moscow Russia 119899 oscow 119899, Russia Russian Acad Med Sci, Sci Ctr Mental Hlth, Moscow 113152, Russia Russian Acad Med Sci Moscow Russia 113152 al Hlth, Moscow 113152, Russia
Titolo Testata:
BIOORGANIC CHEMISTRY
fascicolo: 2, volume: 28, anno: 2000,
pagine: 57 - 61
SICI:
0045-2068(200004)28:2<57:AOMMTA>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
RECEPTORS; CELLS;
Keywords:
nerve growth factor receptors; platelet; ligand-receptor interaction; Scatchard plot; mathematical modeling;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Physical, Chemical & Earth Sciences
Citazioni:
11
Recensione:
Indirizzi per estratti:
Indirizzo: Gurevich, KG Moscow MV Lomonosov State Univ, Lenins Hills, Moscow 119899, Russia Moscow MV Lomonosov State Univ Lenins Hills Moscow Russia 119899
Citazione:
K.G. Gurevich et al., "Application of mathematical modeling to analysis of nerves growth factor interaction with platelets", BIOORG CHEM, 28(2), 2000, pp. 57-61

Abstract

In our previous work (N. D. Chekalina et al. Bull. Exp. Biol. Med., 1996, 121, 295-297 [Russian]) we demonstrated the impossibility of applying Scatchard plots to analysis of the interaction of nerve growth factor (NGF) and platelet due to the nonlinearity of these plots. In the present study we assumed that the cause of nonlinear Scatchard plots is a nonequilibrium interaction of NGF and platelets. With this assumption we used mathematical modeling to define the parameters of the interaction of NGF with platelets. We show that our assumption makes it possible to describe experimental data with a maximal error of 7% using a model with one type of noncooperative binding site and one type of ligand. Due to the maximal error value we did not take into account more complicated models of ligand-receptor interaction. The conjugate gradient method was used to define the parameters of NGF interaction with platelets. The concentration of binding sites found is 18.7 fmol/10(6) cells, and the dissociation constant is 38.4 nM. (C) 2000 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 19:15:25