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Titolo:
Smooth muscle cell matrix metalloproteinase production is stimulated via alpha(v)beta(3) integrin
Autore:
Bendeck, MP; Irvin, C; Reidy, M; Smith, L; Mulholland, D; Horton, M; Giachelli, CM;
Indirizzi:
Univ Toronto, Dept Lab Med & Pathobiol, Toronto, ON M5S 1A8, Canada Univ Toronto Toronto ON Canada M5S 1A8 obiol, Toronto, ON M5S 1A8, Canada Univ Toronto, Dept Med, Toronto, ON M5S 1A8, Canada Univ Toronto Toronto ON Canada M5S 1A8 t Med, Toronto, ON M5S 1A8, Canada St Michaels Hosp, Div Cardiol, Terrence Donnelly Res Labs, Toronto, ON M5B1W8, Canada St Michaels Hosp Toronto ON Canada M5B 1W8 bs, Toronto, ON M5B1W8, Canada Univ Washington, Dept Pathol, Seattle, WA 98195 USA Univ Washington Seattle WA USA 98195 , Dept Pathol, Seattle, WA 98195 USA Royal Free & Univ Coll London, Sch Med, Dept Med, Bone Mineral Ctr, London, England Royal Free & Univ Coll London London England neral Ctr, London, England
Titolo Testata:
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
fascicolo: 6, volume: 20, anno: 2000,
pagine: 1467 - 1472
SICI:
1079-5642(200006)20:6<1467:SMCMMP>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
NEOINTIMAL HYPERPLASIA; GROWTH-FACTOR; ARTERIAL INJURY; RAT OSTEOCLASTS; VASCULAR INJURY; IN-VITRO; ALPHA-V-BETA-3; MIGRATION; OSTEOPONTIN; EXPRESSION;
Keywords:
smooth muscle cells; matrix metalloproteinases; alpha(v)beta(3) integrins; migration; arterial injury;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Bendeck, MP Univ Toronto, Dept Lab Med & Pathobiol, Med Sci Bldg,Room 6315,1 Kings Coll Circle, Toronto, ON M5S 1A8, Canada Univ Toronto Med Sci Bldg,Room 6315,1 Kings Coll Circle Toronto ON Canada M5S 1A8
Citazione:
M.P. Bendeck et al., "Smooth muscle cell matrix metalloproteinase production is stimulated via alpha(v)beta(3) integrin", ART THROM V, 20(6), 2000, pp. 1467-1472

Abstract

This study tests the hypothesis that alpha(V)beta(3) integrin receptors play a critical role in smooth muscle cell (SMC) migration after arterial injury and facilitate migration through the upregulation of matrix metalloproteinase (MMP) activity. We showed that beta(3) integrin mRNA was upregulatedby SMCs in the balloon-injured rat carotid artery in coincidence with MMP-1 expression and early SMC migration. Treatment with the beta(3) integrin-blocking antibody F11 significantly decreased SMC migration into the intima at 4 days after injury, from 110.8+/-30.8 cells/mm(2) in control rats to 10.29+/-7.03 cells/mm(2) in F11-treated rats (P=0.008). By contrast, there was no effect on medial SMC proliferation or on medial SMC number in the carotid artery at 4 days. In vitro, we found that human newborn SMCs produced MMP-1 but that adult SMCs did not. This was possibly due to the fact that newborn SMCs expressed alpha(V)beta(3) integrin receptors, whereas adult SMCsdid not, Stimulation of newborn (alpha(V)beta(3)+) SMCs with osteopontin, a matrix ligand for alpha(V)beta(3), increased MMP-1 production from 114.4+/-35.8 ng/lmL at 0 nmol/L osteopontin to 232.5+/-57.5 ng/mL at 100 nmol/L osteopontin, Finally, we showed that stimulation of newborn SMCs with platelet-derived growth factor-BE and osteopontin together increased the SMC production of MMP-9. Thus, our results support the hypothesis that SMC alpha(V)beta(3) integrin receptors play an important role in regulating migration by stimulating SMC MMP production.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/07/20 alle ore 10:56:09