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Titolo:
An N-terminal peptide from link protein can stimulate biosynthesis of collagen by human articular cartilage
Autore:
Liu, HX; McKenna, LA; Dean, MF;
Indirizzi:
Univ London, Univ London Royal Vet Coll, Dept Vet Basic Sci, London NW1 0TU, England Univ London London England NW1 0TU et Basic Sci, London NW1 0TU, England Univ Erlangen Nurnberg, Inst Pathol, D-91054 Erlangen, Germany Univ Erlangen Nurnberg Erlangen Germany D-91054 -91054 Erlangen, Germany
Titolo Testata:
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
fascicolo: 1, volume: 378, anno: 2000,
pagine: 116 - 122
SICI:
0003-9861(20000601)378:1<116:ANPFLP>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
FIBROBLAST GROWTH-FACTOR; FIBRONECTIN FRAGMENTS; PROTEOGLYCAN BIOSYNTHESIS; IN-VITRO; B-CELLS; CHONDROCYTES; AGE; TISSUE; MATRIX; EXPRESSION;
Keywords:
cartilage; link protein peptide; collagen proteoglycan;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
48
Recensione:
Indirizzi per estratti:
Indirizzo: Dean, MF Univ London, Univ London Royal Vet Coll, Dept Vet Basic Sci, Royal Coll St, London NW1 0TU, England Univ London Royal Coll St London England NW1 0TU W1 0TU, England
Citazione:
H.X. Liu et al., "An N-terminal peptide from link protein can stimulate biosynthesis of collagen by human articular cartilage", ARCH BIOCH, 378(1), 2000, pp. 116-122

Abstract

Previous studies have shown that a peptide identical in sequence to the N-terminal of link protein can function as a growth factor and up-regulate proteoglycan synthesis by human articular cartilage in explant culture (L. A. McKenna et al., Arthritis Rheum. 41, 157-162, 1998). The present study hasextended these investigations to determine the effects of this peptide on the synthesis of collagen, another essential component of normal cartilage matrix. Explants from normal adult knee cartilage were maintained for periods of up to 8 days in medium with or without serum. Peptides were added during each day of culture. Synthesis of collagen was determined by the incorporation of [H-3]proline into hydroxyproline and proteoglycans by incorporation of [S-35]sulfate, The type of newly synthesized collagen was measured by SDS-polyacrylamide gel electrophoresis, fluorography, and immunoblotting. The link protein peptide stimulated synthesis of type II collagen in cartilage from a number of different subjects. Maximum up-regulation of synthesis was attained at a concentration of 100 ng/ml, similar to that observed previously for up-regulation of proteoglycan. Synthesis was up-regulated in both the presence and the absence of serum, although the overall rate of synthesis was greater when serum was added. The findings that this link peptide growth factor stimulated synthesis of proteins, including collagen, in a manner analogous to that shown previously for proteoglycans support the hypothesis that this peptide may have an important role in the feedback control of cartilage matrix synthesis. (C) 2000 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/09/20 alle ore 15:57:20