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Titolo:
Effects of lipid-derivatized glycosaminoglycans (GAGs), a novel probe for functional analyses of GAGs, on cell-to-substratum adhesion and neurite elongation in primary cultures of fetal rat hippocampal neurons
Autore:
Oohira, A; Kushima, Y; Tokita, Y; Sugiura, N; Sakurai, K; Suzuki, S; Kimata, K;
Indirizzi:
Inst Dev Res, Dept Perinatol & Neuroglycosci, Kasugai, Aichi 4800392, Japan Inst Dev Res Kasugai Aichi Japan 4800392 i, Kasugai, Aichi 4800392, Japan Seikagaku Corp, Tokyo Res Inst, Tokyo 2070021, Japan Seikagaku Corp Tokyo Japan 2070021 Tokyo Res Inst, Tokyo 2070021, Japan Aichi Med Univ, Inst Mol Sci Med, Nagakute, Aichi 4801195, Japan Aichi MedUniv Nagakute Aichi Japan 4801195 agakute, Aichi 4801195, Japan
Titolo Testata:
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
fascicolo: 1, volume: 378, anno: 2000,
pagine: 78 - 83
SICI:
0003-9861(20000601)378:1<78:EOLG(A>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
CHONDROITIN SULFATE PROTEOGLYCAN; EXTRACELLULAR-MATRIX; NERVOUS-SYSTEM; PC12D CELLS; BRAIN; OUTGROWTH; GROWTH; FIBRONECTIN; PROTEINS; L1;
Keywords:
glycosaminoglycan; chondroitin sulfate; hyaluronic acid; neuron; neurite elongation; cell attachment;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
26
Recensione:
Indirizzi per estratti:
Indirizzo: Oohira, A Inst Dev Res, Dept Perinatol & Neuroglycosci, Kasugai, Aichi 4800392, Japan Inst Dev Res Kasugai Aichi Japan 4800392 , Aichi 4800392, Japan
Citazione:
A. Oohira et al., "Effects of lipid-derivatized glycosaminoglycans (GAGs), a novel probe for functional analyses of GAGs, on cell-to-substratum adhesion and neurite elongation in primary cultures of fetal rat hippocampal neurons", ARCH BIOCH, 378(1), 2000, pp. 78-83

Abstract

The effects of glycosaminoglycans (GAG) on cell-to-substratum adhesion andneurite elongation were examined in primary cultures of fetal rat hippocampal neurons using tissue culture dishes coated with GAGs coupled to dipalmitoylphosphatidylethanolamine (PE), a novel probe for biological functions of GAGs. Both chondroitin sulfate conjugate to PE (CS-PE) and hyaluronic acid conjugate to PE (HA-PE) promoted neurite elongation from neurons in a dose-dependent manner when immobilized onto polylysine-coated dishes at various concentrations up to 1.0 mu g/ml. The coating of CS-PE or HA-PE at a concentration higher than 1.0 mu g/ml resulted in failure of neurite extension and adhesion of neurons to the substrata. In contrast, heparin conjugate toPE (HP-PE) did not exert any effects on neurite elongation or on cell attachment at these concentrations. These findings suggest that GAGs serve as amodulator for neurite elongation during neuronal network formation in the developing central nervous system. (C) 2000 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 18:49:28