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Titolo:
Cardioplegia-induced cell swelling: Prevention by normothermic infusion
Autore:
Stephenson, ER; Jayawant, AM; Baumgarten, CM; Damiano, RJ;
Indirizzi:
Penn State Geisinger Hlth Syst, Milton S Hershey Med Ctr, Sect Cardiothorac & Vasc Surg, Hershey, PA USA Penn State Geisinger Hlth Syst Hershey PA USA Vasc Surg, Hershey, PA USA
Titolo Testata:
ANNALS OF THORACIC SURGERY
fascicolo: 5, volume: 69, anno: 2000,
pagine: 1393 - 1398
SICI:
0003-4975(200005)69:5<1393:CCSPBN>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
HYPOTHERMIC CARDIOPLEGIA; VENTRICULAR MYOCYTES; MYOCARDIAL-ISCHEMIA; BLOOD CARDIOPLEGIA; RABBIT ATRIAL; EDEMA; VOLUME; ARREST; HEART; PROTECTION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
25
Recensione:
Indirizzi per estratti:
Indirizzo: Damiano, RJ Washington Univ, Med Ctr, Div Cardiothorac Surg, 1 Barnes Jewish Hosp Plaza,Suite 3108 Quceny Towe, St Louis, MO 63110 USA Washington Univ 1 Barnes Jewish Hosp Plaza,Suite 3108 Quceny Towe St Louis MO USA 63110
Citazione:
E.R. Stephenson et al., "Cardioplegia-induced cell swelling: Prevention by normothermic infusion", ANN THORAC, 69(5), 2000, pp. 1393-1398

Abstract

Background. Previous work has shown significant swelling of isolated rabbit myocytes exposed to cold hyperkalemic cardioplegia; however, the effect of warm hyperkalemic cardioplegia on myocyte volume is unknown. This study examined the effect of warm hyperkalemic cardioplegia (St. Thomas' solution)on myocyte volume. Methods. Myocytes were enzymatically isolated and placed on an inverted video microscope. Tyrode's solution (37 degrees C) was infused for 10 minutesto establish baseline cell volumes. Subsequently, either the control Tyrode's or St. Thomas' was infused either at 37 degrees C and 9 degrees C respectively (n = 5 for all groups) for 20 minutes, followed by a 30-minute reperfusion with 37 degrees C Tyrode's. Cell volume was determined from cell images captured every 5 minutes. Results. Myocyte swelling occurred rapidly on exposure to cold St. Thomas'solution to a maximum of 9.8 +/- 2.1% (p < 0.001). In contrast, myocytes exposed to warm cardioplegia did not show any volume changes during exposureto cardioplegia. However, upon reexposure to Tyrode's, these cells showed shrinkage below their baseline volume (p < 0.001). Conclusions. The cell swelling associated with hypothermic cardioplegia isprevented by normothermic infusion. (C) 2000 by The Society of Thoracic Surgeons.

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Documento generato il 27/09/20 alle ore 21:35:46