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Titolo:
MIB-1 immunohistometry of follicular adenoma and follicular carcinoma of the thyroid gland
Autore:
Rickert, D; Mittermayer, C; Lindenfelser, R; Biesterfeld, S;
Indirizzi:
Aachen Tech Univ, Inst Pathol, D-52074 Aachen, Germany Aachen Tech Univ Aachen Germany D-52074 Pathol, D-52074 Aachen, Germany Aachen Tech Univ, Dept Otorhinolaryngol, D-52074 Aachen, Germany Aachen Tech Univ Aachen Germany D-52074 aryngol, D-52074 Aachen, Germany Knappschafts Krankenhaus Bardenberg, Inst Pathol, Bardenberg, Germany Knappschafts Krankenhaus Bardenberg Bardenberg Germany denberg, Germany
Titolo Testata:
ANALYTICAL AND QUANTITATIVE CYTOLOGY AND HISTOLOGY
fascicolo: 3, volume: 22, anno: 2000,
pagine: 229 - 234
SICI:
0884-6812(200006)22:3<229:MIOFAA>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
CELL NUCLEAR ANTIGEN; MONOCLONAL-ANTIBODY KI-67; NEGATIVE BREAST-CANCER; PROGNOSTIC VALUE; PROLIFERATIVE ACTIVITY; GASTRIC-CARCINOMA; EXPRESSION; PCNA; TUMORS; APOPTOSIS;
Keywords:
thyroid diseases; thyroid neoplasms; image analysis; computer-assisted; immunohistochemistry; immunohistometry; MIB-1;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Biesterfeld, S Aachen Tech Univ, Inst Pathol, Pauwelsstr 30, D-52074 Aachen, Germany Aachen Tech Univ Pauwelsstr 30 Aachen Germany D-52074 many
Citazione:
D. Rickert et al., "MIB-1 immunohistometry of follicular adenoma and follicular carcinoma of the thyroid gland", ANAL QUAN C, 22(3), 2000, pp. 229-234

Abstract

OBJECTIVE: To analyze cellular proliferative activity, MIB-1 immunopositivity of normal tissue (n = 20), follicular adenoma (n=30) and follicular carcinoma (n = 32) of the thyroid gland was analyzed by means of immunohistometry. STUDY DESIGN: Immunohistochemical reactions were performed on 3-mu m sections from routinely formalin fixed and paraffin embedded surgical specimens using an indirect peroxidase method. The rate of immunostained cells was determined using the CM-2 TV image analysis system (Hund, Wetzlar, Federal Republic of Germany). Forty viewing fields (1.94 mm(2)) were measured with 20:1 objective magnification. An average of 5,965 cells were assessed in eachcase. RESULTS: Mean MIB-1 immunopositivity was higher in follicular carcinoma (average, 2.30%) and follicular adenoma (0.58%) than in normal thyroid tissue(0.14%). The distribution of single values differed significantly be tweengroups (P <.001). To test the suitability of MIB-1 immunohistometry for the differential diagnosis of follicular adenoma and follicular carcinoma, different four-field tables with varying thresholds were calculated. Using a threshold of 0.9%,follicular carcinoma could be detected with a sensitivityof 75% (24/32) and a specificity of 83% (25/30). If a specificity of 90% is required (27/30), the sensitivity of the test decreases to 69% (22/32), based on a threshold of 1.1%. CONCLUSION: As some overlap of single values has to be considered MIB-1 immunohistometry, although presenting new insights into the proliferative potential of thyroid lesions, is of only limited value for the differential diagnosis of follicular lesions in routine surgical pathology.

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Documento generato il 01/12/20 alle ore 07:29:25