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Titolo:
Adhesion of uric acid crystals to the surface of renal epithelial cells
Autore:
Koka, RM; Huang, E; Lieske, JC;
Indirizzi:
Univ Chicago, Dept Med, Chicago, IL 60637 USA Univ Chicago Chicago IL USA60637 hicago, Dept Med, Chicago, IL 60637 USA
Titolo Testata:
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY
fascicolo: 6, volume: 278, anno: 2000,
pagine: F989 - F998
SICI:
0363-6127(200006)278:6<F989:AOUACT>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
CALCIUM-OXALATE NEPHROLITHIASIS; MONOSODIUM URATE; ERYTHROCYTE-MEMBRANES; MONOHYDRATE CRYSTALS; STONE DISEASE; PROTEIN; UROLITHIASIS; NEPHROCALCIN; INHIBITION; INVITRO;
Keywords:
BSC-1 cells; glycosaminoglycans; Madin-Darby canine kidney cells; nephrolithiasis; 3T3 fibroblasts;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Lieske, JC Mayo Clin & Mayo Fdn, Div Nephrol, 200 1st St SW, Rochester, MN55905 USA Mayo Clin & Mayo Fdn 200 1st St SW Rochester MN USA 55905 5 USA
Citazione:
R.M. Koka et al., "Adhesion of uric acid crystals to the surface of renal epithelial cells", AM J P-REN, 278(6), 2000, pp. F989-F998

Abstract

Adhesion of microcrystals that nucleate in tubular fluid to the apical surface of renal tubular cells could be a critical step in the formation of kidney stones, 12% of which contain uric acid (UA) either alone or admixed with calcium oxalates or calcium phosphates. UA crystals bind rapidly to monolayer cultures of monkey kidney epithelial cells (BSC-1 line), used to model the surface of the nephron, in a concentration-dependent manner. The urinary glycoproteins osteopontin, nephrocalcin, and Tamm-Horsfall glycoproteinhad no effect on binding of UA crystals to the cell surface, whereas otherpolyanions including specific glycosaminoglycans blocked UA crystal adhesion. Specific polycations also inhibited adhesion of UA crystals and appeared to exert their inhibitory effect by coating cells. However, removal of anionic cell surface molecules with neuraminidase, heparitinase I, or chondroitinase ABC each increased UA crystal binding, and sialic acid-binding lectins had no effect. These observations suggest that hydrogen bonding and hydrophobic interactions play a major role in adhesion of electrostatically neutral UA crystals to renal cells, unlike the interaction of calcium-containing crystals with negatively charged molecules on the apical cell surface via ionic forces. After adhesion to the plasma membrane, subsequent cellularevents could contribute to UA crystal retention in the kidney and the development of UA or mixed calcium and UA calculi.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/03/20 alle ore 19:42:39